文献类型：期刊文献

中文题名：大孔吸附树脂分离纯化肉苁蓉中苯乙醇苷工艺研究

英文题名：Optimization of the Isolation and Purification Technology of Phenylethanoid Glycosides from Cistanche Tubulosa by Macroporous Adsorption Resin

作者：顾家珲[1];余晓晖[1,2];吴蓉[1];赵信科[3];赵磊[1,2]

第一作者：顾家珲

机构：[1]甘肃中医学院药学系;[2]甘肃省高校中藏药化学与质量研究省级重点实验室;[3]兰州铝厂职工医院

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2011

卷号：28

期号：11

起止页码：1006

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：肉苁蓉;苯乙醇苷;大孔吸附树脂;纯化

外文关键词：Cistanche tubulosa; phenylethanoid glycosides; macroporous adsorption resin; purification

摘要：目的筛选富集肉苁蓉中苯乙醇苷的最佳树脂和工艺条件,使分离纯化工艺达到最优化。方法选择7种大孔吸附树脂(AB-8、DM301、DM130、D1400、DA-201、HPD-100、D-101-I),通过树脂对苯乙醇苷的静态吸附和解析能力,筛选出最佳树脂,再通过树脂的静态特性和动态特性的筛选优化工艺。结果 DA-201的吸附解析最佳条件为:上样流速为1.5 mL.min-1,洗脱剂为90%的乙醇。按最佳工艺苯乙醇苷的纯度由25.1%上升为51.2%。结论该法简便、可行,为肉苁蓉中苯乙醇苷的纯化提供了参考。
OBJECTIVE To investigate the optimal macroporous adsorption resin and process to separate phenylethanoid glycosides from Cistanche tubulosa.METHODS Seven types of macroporous adsorption resin were evaluated for separating efficiency by measuring the adsorption ratio,eluting ratio of phenylethanoid glycosides.RESULTS The optimal technologies of DA-201 macroprous dosorption resin were as follows： the simple flow was 1.5 mL·min-1,the column was washed by 95% ethanol.After enrichment and purification,the product purity of phenylethanoid glycosides was up to 51.2%.CONCLUSION This method is simple,feasible and can provide useful reference for the isolation and purification of phenylethanoid glycosides from Cistanche tubulosa.