文献类型：期刊文献

中文题名：小儿平喘方对哮喘大鼠血清白细胞介素-4、γ-干扰素水平的影响

英文题名：The influence of Xiaoer Pingchuan Fang on IL-4 and IFN-γ levels of the serum in asthmatic rats

作者：吴尚华[1];吴丽萍[2];李玉霞[2];尚菁[3];薛付春[3]

第一作者：吴尚华

机构：[1]甘肃省中医院白银分院门诊部,甘肃白银730900;[2]甘肃中医药大学附属医院儿科,甘肃兰州730020;[3]甘肃中医药大学中医临床学院,甘肃兰州730000

第一机构：甘肃省中医院白银分院门诊部,甘肃白银730900

年份：2015

卷号：11

期号：4

起止页码：5

中文期刊名：中医儿科杂志

外文期刊名：Journal of Pediatrics of Traditional Chinese Medicine

基金：2014年甘肃中医药大学附属医院院内科研基金一般项目

语种：中文

中文关键词：小儿平喘方;支气管哮喘;白细胞介素-4;γ-干扰素;实验研究

外文关键词：Xiaoer Pingchuan Fang ; bronchial asthma; IL-4 ; IFN-γ; experimental research

摘要：目的观察小儿平喘方对哮喘模型大鼠血清中γ-干扰素(IFN-γ)、白细胞介素-4(IL-4)含量、IFN-γ/IL-4比值的影响,从而探讨小儿平喘方治疗支气管哮喘的药效机制。方法选取SD大鼠32只,随机分为4组:正常对照组、哮喘模型组、小儿平喘方小剂量组(小剂量组)和小儿平喘方大剂量组(大剂量组),每组8只,采用卵清蛋白(OVA)致敏和雾化吸入激发制备大鼠哮喘模型,用双位点夹心酶联免疫吸附试验测定各组大鼠血清中IL-4和IFN-γ含量。结果与正常对照组比较,哮喘模型组大鼠血清IFN-γ的含量与IFN-γ/IL-4比值均降低(P<0.05),而IL-4的含量则显著升高(P<0.05);与哮喘模型组比较,大、小剂量组大鼠血清IFN-γ的含量与IFN-γ/IL-4比值均显著升高(P<0.01),而IL-4的含量则降低(P<0.05)。结论小儿平喘方能显著降低支气管哮喘模型大鼠血清IL-4的含量,提高IFN-γ的含量及失衡的IFN-γ/IL-4比值,从而调节免疫功能,减轻炎症反应,控制哮喘的发作。
Objective To observe the influence of Xiaoer Pingchuan Fang on IL-4 and IFN-γ levels and the IFN-γ/IL-4 ratio of the serum in asthmatic rats, so as to investigate the pharmacodynamic mechanism of Xiaoer Pingchuan Fang in treating children bronchial asthma. Methods 32 SD rats were randomly divided into 4 groups ： a normal control group, an asthma model group, a Xiaoer Pingchuan Fang group of low dose and a Xiaoer Pingchuan Fang group of large dose, 8 each. The ovalbumin （OVA） sensitization and the atomization inhalation were used to motivate and prepare for models of asthma rats, with the use of double loci enzyme combined with the immunosorbent experiment to determine the IL-4 and IFN-γcontent of the rat serum in each group. Results Compared with those in the normal control group, the IFN-γ content and the IFN-γ /IL-4 ratio in the asthma model group were both lowered（P〈0.05 ）, while the content of IL-4 increased（P〈0.05）. Compared with those in the asthma model group, both the IFN-γ content and the IFN-γ/IL-4 ratio in the Xiaoer Pingchuan Fang group of low dose and the Xiaoer Pingchuan Fang group of large dose significantly increased（P〈0.01 ） , but the content of IL-4 increased（ P 〈 0. 05 ）. Conclusion Xiaoer Pingchuan Fang can significantly reduce the content of IL-4 in bronchial asthma rats serum and increase the content of IFN-γ and imbalanced ratio of IFN-γ IL-4, accordingly regulating the immune function, reducing inflammation reactions and controlling the asthma attacks.