文献类型：期刊文献

中文题名：归芪益元膏对重离子辐射旁效应损伤大鼠的影响

英文题名：Study on Protective Effects of Guiqi Yiyuan Ointment on Rats Damaged Caused by Heavy Ion Radiation Induced Bystander Effects

作者：张朝宁[1];李金田[1];刘永琦[1];蔺兴遥[1];李雪燕[1];孙少伯[1];李娟[1];王萌[1];陈继尧[1]

第一作者：张朝宁

机构：[1]甘肃中医药大学

第一机构：甘肃中医药大学

年份：2017

卷号：24

期号：7

起止页码：45

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金(81460695)

语种：中文

中文关键词：辐射旁效应;重离子;归芪益元膏;转化生长因子-β1;白细胞介素-6;白细胞介素-1β;大鼠;雄性

外文关键词：radiation induced bystander effects; heavy ion; Guiqi Yiyuan Ointment; TGF-β1; IL-6; IL-1β; rats; male

摘要：目的观察归芪益元膏对重离子辐射旁效应损伤大鼠的影响,探讨其可能的作用机制。方法将42只SPF级Wistar雄性大鼠随机分为空白对照组、单纯辐射组和归芪益元膏组。归芪益元膏组预先给予归芪益元膏灌胃2周,单纯辐射组和归芪益元膏组大鼠右肺予2 Gy ^(12)C^(6+)离子束单次照射,空白对照组不予照射。照射后6、12、24 h处死各组大鼠,检测外周血象及白细胞介素(IL)-6、转化生长因子-β1(TGF-β1)、IL-1β含量,HE染色观察大鼠肺组织病理形态。结果与空白对照组比较,照射后12 h单纯辐射组大鼠血清IL-6、TGF-β1、IL-1β含量明显升高(P<0.01),外周血白细胞、红细胞、血小板、血红蛋白明显下降(P<0.01);HE染色显示照射后24 h大鼠肺泡壁增厚,肺泡腔内有渗出物及炎性细胞浸润等病理改变。与单纯辐射组12 h比较,归芪益元膏组12 h大鼠IL-6、TGF-β1、IL-1β含量明显下降(P<0.01),血常规各项指标显著上升(P<0.01),大鼠肺组织病理损伤有一定的改善。结论归芪益元膏对重离子辐射旁效应损伤具有防护作用。
Objective To discuss the protective effects of Guiqi Yiyuan Ointment on damages caused by heavy ion radiation induced bystander effects; To explore the possible mechanism. Methods Totally 42 Wistar male rats were randomly divided into blank control group, pure radiation group and Guiqi Yiyuan Ointment group. The Guiqi Yiyuan Ointment group was given Guiqi Yiyuan Ointment by gavage for two weeks in advance. Later the right lungs of the rats in the pure radiation group and Guiqi Yiyuan Ointment group were radiated once by 2 Gy 12C6＋, while the blank control group received no radiation. 6, 12, 24 h after radiation all groups of rats were executed. Peripheral hemogram and the levels of IL-6, TGF-β1 and IL-1β in serum of the rats were examined. The changes of lung tissue pathology morphology in the rats were observed by HE staining. Results Compared with the blank control group, the contents of IL-6, TGF-β1 and IL-1β in serum of the pure radiation group increased obviously at 12 h after radiation （P〈0.01）, and the amount of leukocyte, erythrocyte, blood platelet and hemoglobin distinctly declined at 12 h after radiation （P〈0.01）; HE staining showed that the alveolar wall was thickened at 24 h after radiation, and there were exudate and inflammatory cell infiltration in the alveolar cavity. Compare with the pure radiation group at 12 h after radiation, the levels of IL-6, TGF-β1 and IL-1β in Guiqi Yiyuan Ointment group decreased significantly at 12 h after radiation （P〈0.01）. Indexes of blood routine significantly increased （P〈0.01）, and the pathological changes of lung tissue in rats improved （P〈0.01）. Conclusion Guiqi Yiyuan Ointment can protect damages caused by heavy ion radiation induced bystander effects.