文献类型：期刊文献

英文题名：COL8A1 Predicts the Clinical Prognosis of Gastric Cancer and Is Related to Epithelial-Mesenchymal Transition

作者：She, Yali[1,2];Zhao, Xiaowen[1,3,4];Wu, Pingfan[1,3,4];Xue, Ling[1,3];Wan, Shengfang[1];Zhang, Lei[1];Li, Changtian[1];Cai, Hui[5,6];Li, Yaling[1,2]

第一作者：舍雅莉

通信作者：Li, YL[1];Li, YL[2];Cai, H[3];Cai, H[4]

机构：[1]Gansu Univ Chinese Med, Sch Basic Med, Dept Pathol, Lanzhou, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis & Study Prev, Lanzhou, Gansu, Peoples R China;[3]Chinese Peoples Liberat Army, Joint Logist Support Unit, Hosp 940, Dept Pathol, Lanzhou, Gansu, Peoples R China;[4]Chinese Peoples Liberat Army, Hosp Joint Logist Support Force 940, Lab Preclin Med, Key Lab Stem Cells & Gene Drug Gansu Prov, Lanzhou, Gansu, Peoples R China;[5]Gansu Prov Hosp, Key Lab Mol Diagnost & Precis Med Surg Oncol Gans, Lanzhou 730000, Gansu, Peoples R China;[6]Gansu Prov Hosp, NHC Key Lab Diag & Therapy Gastrointestinal Tumor, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Basic Med, Dept Pathol, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis & Study Prev, Lanzhou, Gansu, Peoples R China;[3]corresponding author), Gansu Prov Hosp, Key Lab Mol Diagnost & Precis Med Surg Oncol Gans, Lanzhou 730000, Gansu, Peoples R China;[4]corresponding author), Gansu Prov Hosp, NHC Key Lab Diag & Therapy Gastrointestinal Tumor, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2022

卷号：2022

外文期刊名：BIOMED RESEARCH INTERNATIONAL

收录：;Scopus(收录号：2-s2.0-85133250073);WOS:【SCI-EXPANDED(收录号:WOS:001153387600001)】；

基金：We acknowledged the grants from all the institutions and departments we mentioned in our funding supports. The authors are thankful for the data provided by GEO and TCGA. This study was supported by the Colleges and Universities Innovation Ability Improvement Project of Gansu Province, China (No. 2021B-166); Double First-Class Scientific Research Key Projects in Gansu Province (No. GSSYLXM-21); Central to Guide Local Scientific and Technological Development; Major Research Projects on the Prevention of Chronic Non-Communicable Diseases (No. 2018YFC1311506); Science and Technology Planning Project of Chengguan District, Lanzhou City, Gansu Province, China (No. 2021-2-11); and Wuwei Municipal Science and Technology Plan (No. WW2001012).

语种：英文

摘要：Background. Gastric cancer (GC) is the fifth most common malignant tumor and the third leading cause of cancer-related deaths. Because GC has the characteristics of high heterogeneity, unclear mechanism, limited treatment methods, and low five-year survival rate, it is necessary to find the prognostic biomarkers of GC and explore the mechanism of GC. Methods. We first identified differentially expressed genes (DEGs) between gastric cancer and normal gastric cells through expression analysis. A protein-protein interaction (PPI) network was constructed to find tightly connected modules. We performed survival analysis on the DEGs in the modules to identify genes with prognostic significance. Gene set enrichment analysis (GSEA) was used to identify gene enrichment pathways. Finally, we used our own collected clinical samples of 119 gastric adenocarcinoma (STAD) tissues and 40 normal gastric tissues to perform immunohistochemical (IHC) staining to verify the differential expression of COL8A1 in STAD tissues and normal gastric tissues and its correlation with epithelial-mesenchymal transition- (EMT-) related factors. Results. We identified 356 DEGs through differential expression analysis. Through PPI analysis and survival analysis, we determined that the collagen type VII alpha-1 chain (COL8A1) gene has prognostic significance. GSEA analysis showed that COL8A1 was significantly enriched in the EMT. IHC results showed that COL8A1 was upregulated in STAD tissues and could be used as an independent prognostic factor and was related to EMT. Conclusion. This study shows that COL8A1 is related to the prognosis of GC patients and might affect the progress of GC through the EMT pathway. Therefore, COL8A1 may be a biomarker for predicting the prognosis of GC.