文献类型：期刊文献

中文题名：超高效液相法(UPLC)用于黑果枸杞指纹图谱的研究

英文题名：Analysis of Ultra Performance Liquid( UPLC)for Black Fruit Wolfberry Fingerprints

作者：林丽[1];石亚娜[2];高素芳[1];晋玲[1,3];王振恒[1,3];赵帆[1]

第一作者：林丽

机构：[1]甘肃中医学院药学院;[2]云南省农业科学院药用植物研究所;[3]中(藏)药资源研究所

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2017

卷号：30

期号：6

起止页码：1426

中文期刊名：西南农业学报

外文期刊名：Southwest China Journal of Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金：国家中医药管理局中医药行业专项“甘肃省代表性区域中药资源保护利用”(2012070025-02);甘肃省中医药管理局项目“黑河流域黑果枸杞的资源调查及质量评价”GZK-2012-21;甘肃省科技计划基础研究创新群体“甘肃省中藏药资源评价、保护与开发利用”(1606RJIA323);中央财政引导地方科技创新平台项目“甘肃省中药资源与新产品开发平台建设”(2016-A-02)

语种：中文

中文关键词：UPLC;黑果枸杞;指纹图谱

外文关键词：UPLC ; Lycium ruthenicum Murr; Fingerprint

摘要：【目的】建立黑果枸杞UPLC指纹图谱分析技术,为快速鉴别黑果枸杞提供依据。【方法】采用超高效液相色谱法进行测定。【结果】以ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm);流动相为甲醇(A)、0.5%三氟乙酸、1%甲酸水溶液(B)、乙腈(C),以0.3 m L/min流速进行梯度洗脱,检测波长300 nm,进样量1.000μl,柱温35℃为色谱条件,建立了黑果枸杞的UPLC特征指纹图谱共有模式,标定了13个共有峰,14批次黑果枸杞的相似度为0.809~0.989,不同省份差异较大。【结论】该方法快速、高效,稳定可靠,为黑果枸杞的鉴别和质量评价及质量控制提供了科学依据。
[ Objective] The purpose of this paper is to provide a rapid identification method for Lycium ruthenicum Murr based on the estab-lishment of UPLC fingerprint. [ Method] Experiments were performed by UPLC method. [ Result] Using ACQUITY UPLC BEH C18 column（2.1 mm x 50 mm, 1.7 I^m）, methanol（ A）, 0.5 % trichloroacetic, 1% formic acid （ B ） and acetonitrile （ C ） as the mobile phase, thedetection wavelength 300 nm, column temperature 35 ℃, the flow rate 0.3 mL/min, the injection volume 1. 000 Ixl, the mutual mode ofUPLC fingerprint of Lycium ruthenicum Murr was established. There were 13 common peaks in the fingerprint of 14 samples. The similarityof the 14 samples was ranged from 0. 809 to 0. 989 and it indicated that the UPLC fingerprints of the samples from different provinces wereobviously different. [ ConclusionlThe UPLC method has good precision, stability and repeatability. The fingerprint established in this studycan supply scientific method for the identification, quality evaluation and control of Lycium ruthenicum Murr.