文献类型：期刊文献

英文题名：Studies on the interactions of AFPs and bZIP transcription factor ABI5

作者：Wei, Jinkui[1];Li, Xiaojuan[1];Song, Peng[2];Wang, Yonggang[1];Ma, Jianzhong[1]

第一作者：Wei, Jinkui

通信作者：Ma, JZ[1]

机构：[1]Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China;[2]Gansu Univ Chinese Med, Key Lab Prevent & Treatment Chron Dis TCM Gansu P, Affiliated Hosp, Lanzhou 730000, Peoples R China

第一机构：Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China.

年份：2022

卷号：590

起止页码：75

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：;Scopus(收录号：2-s2.0-85122107182);WOS:【SCI-EXPANDED(收录号:WOS:000737467800010)】；

基金：This work was supported by the Natural Science Foundation of China (grant number 31860063).

语种：英文

外文关键词：ABI5; AFP; Interaction; Transcriptional activity; Arabidopsis

摘要：AFP1 interacts with ABI5 and negatively regulates the abscisic acid (ABA) signaling by accelerating ABI5's degradation during the seed germination phase in Arabidopsis, but the underlying mechanism remains unclear. Moreover, the molecular basis of the interaction between AFP1 homologs and ABI5 has yet to be elucidated. In this study, the patterns of their interactions with ABI5 were investigated in detail. We found that AFP2/3/4 can bind two regions of ABI5, one is ABI51aa to 135aa and another is ABI5202aa to 213aa. However, AFP1 only interacts with the second region of ABI5, i.e. ABI5202aa to 213aa. Prior research has shown that ABI51aa to 135aa is related to the transcriptional activity of ABI5. Thus, our results suggest that AFPs may also modulate ABI5, by directly binding to its transcriptional activation domain, thereby influencing its transcriptional activity. Further, interactions between AFPs and ABI5 were not affected if the Ser42th in the ABI5-SnRK2 motif were mutated respectively to Glu or Ala. Nevertheless, interactions between AFPs and ABI5 were eliminated if the Thr47th and Thr206th of ABI5 were mutated respectively to Glu or Ala. Since the two residues of Thr47th and Thr206th were located in the phosphorylation motifs of CKII, AFPs might regulate the activities of ABI5 transcription factor through a CKII-dependent pathway. (c) 2021 Published by Elsevier Inc.