文献类型：期刊文献

中文题名：模拟高原低氧环境下大鼠性激素水平及红细胞、血红蛋白的变化研究

英文题名：Changes of sex hormone levels,red blood cells and hemoglobin in rats under mimetic plateau hypoxia

作者：郭文静[1,2];罗晓红[2];吴小芬[2];王春雨[2];李静[2];牛廷献[3];冯小明[3];郭晓宇[3]

第一作者：郭文静

机构：[1]甘肃中医药大学,甘肃兰州730050;[2]兰州军区兰州总医院;[3]不详

第一机构：甘肃中医药大学

年份：2016

卷号：0

期号：5

起止页码：321

中文期刊名：西北国防医学杂志

外文期刊名：Medical Journal of National Defending Forces in Northwest China

基金：全军医药卫生科研基金资助项目(CLZ14JB06)

语种：中文

中文关键词：高原低氧;性激素;红细胞计数;血红蛋白含量

外文关键词：plateau hypoxia;;sex hormones;;red blood cell count;;hemoglobin content

摘要：目的:探讨不同海拔梯度低氧环境对大鼠睾酮(T)、雌二醇(E2)、红细胞计数(RBC)、血红蛋白(HGB)的影响。方法:将96只大鼠随机分为4组,即海拔1 500(control)、3 500、5000、6000m,各组进一步分为缺氧12、24、48h,每组8只,共12组。对照组置于海拔1 500m常规饲养,缺氧组大鼠置于模拟不同海拔低压氧舱内,分别放置12、24、48h后取出,经心脏取血采集血液后处死。分离睾丸、附睾组织,称重,计算睾丸、附睾指数。测定RBC、HGB及血清睾酮(T)、雌二醇(E2)含量。结果:与平原对照组比较,大鼠睾丸、附睾指数有下降趋势,但无统计学意义(P>0.05);血清T、E2含量随海拔高度上升而下降,差异有统计学意义(P<0.05);RBC计数、HGB含量随海拔高度上升呈升高趋势,差异有统计学意义(P<0.05)。结论:随着海拔梯度升高,性激素分泌减少,提示缺氧可能抑制了性腺功能。RBC计数、HGB含量升高是机体的一种适应性和代偿性改变。
Objective:To explore the effects of plateau hypoxia under different altitudes on testosterone,estradiol,red blood cell count and hemoglobin changes in rats.Methods:Ninety-six Wistar rats were randomly divided into four groups of different altitudes,namely,1 500m(control),3 500 m,5000mand6000m.Each group was further divided into four subgroups with 12 h,24hand 48 hhypoxia duration.Thus,there were a total of 12 groups with 8rats in each.The rats in the control group were fostered regularly at the altitude of 1 500 m while the hypoxia groups were fostered in chambers simulating plateau hypoxia with different altitudes.After hypoxia exposure for 12 h,24hand 48 h,the rats were executed after heart blood collection.The testis and epididymis tissue were separated and weighed in order to calculate the testis and epididymis indexes.The RBC,HGB,serum testosterone(T)and estradiol(E2)levels were measured.Results:Compared with those in the control group,the testis and epididymis indexes showed a tendency of decrease with no statistical significance.The levels of T and E2 revealed a statistically significant decline while the levels of RBC and HGB increased significantly with the elevation of altitude(P<0.05).Conclusion:With the elevation of altitude,the secretion of sex hormones decreased,indicating that hypoxia may inhibit the function of sex gland.The increase of RBC counts and HGB shows an adaptable and compensatory change of body.