文献类型：期刊文献

中文题名：黄芪多糖抑制黄嘌呤氧化酶诱导的肺癌A549细胞自噬并调节自噬相关蛋白的表达

英文题名：Astragalus polysaccharide inhibits autophagy and regulates expression of autophagy-related proteins in lung cancer A549 cells induced by xanthine oxidase

作者：王雪林[1,2,3];李杨[1];刘丹[1,2,3];王彦君[1,2,3];明海霞[1,2,3,4]

第一作者：王雪林

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学甘肃省中药药理与毒理学重点实验室,甘肃兰州730000;[3]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究重点实验室,甘肃兰州730000;[4]甘肃中医药大学中西医结合研究所,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2019

卷号：35

期号：7

起止页码：619

中文期刊名：细胞与分子免疫学杂志

外文期刊名：Chinese Journal of Cellular and Molecular Immunology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；PubMed;

基金：甘肃省中医药管理局资助项目(GZK-2017-6);甘肃省高等学校科研项目(2018A-046);甘肃省高校重大疾病分子医学与中医药防治研究重点实验室开放基金(FZYX17-18-9);甘肃中医药大学研究生创新基金(CX201833)

语种：中文

中文关键词：黄芪多糖;A549细胞;自噬;微管相关蛋白1轻链3B(LC3B);beclin1;哺乳动物雷帕霉素靶蛋白(mTOR)

外文关键词：Astragalus polysaccharide;A549 cells;autophagy;LC3B;beclin1;mammalian target of rapamycin(mTOR)

摘要：目的探讨黄芪多糖(APS)对黄嘌呤氧化酶(XOD)诱导的肺癌A549细胞的自噬作用及微管相关蛋白1轻链3B(LC3B)、哺乳动物雷帕霉素靶蛋白(mTOR)、beclin1蛋白表达的影响。方法 A549细胞分为对照组、模型组、100μg/mL APS处理组、200μg/mL APS处理组及400μg/mL APS处理组,除对照组,其余4组均需XOD诱导24 h建立自噬模型。采用透射电镜观察自噬体的形态、单丹磺酰尸胺(MDC)染色荧光显微镜观察自噬体数量的变化, Western blot法检测LC3B、mTOR、beclin1蛋白水平。结果与空白组比较,模型组细胞自噬体数量增多,且LC3B、beclin1蛋白水平升高, mTOR蛋白水平降低;与模型组比较,(200、400)μg/mL APS处理组细胞自噬体数量显著减少, LC3B、beclin1蛋白水平降低, mTOR蛋白水平升高。结论 APS可降低XOD诱导的A549肺癌细胞自噬水平,并降低LC3B、beclin1蛋白表达、增加mTOR蛋白表达。
Objective To investigate the effects of Astragalus polysaccharide(APS) on autophagy and expression of microtubule-associated protein 1 light chain 3B(LC3B), mammalian target of rapamycin(mTOR) and beclin1 in xanthine oxidase(XOD)-induced autophagic model of non-small cell lung cancer A549 cells. Methods A549 cells were divided into five groups: control group, model group, 100, 200 and 400 μg/mL APS-treated group. Except for control group, all groups were administered XOD for 24 hours to establish autophagic models. Morphology of autophagosome was detected by transmission electron microscopy(TEM) and the number was counted by monodansylcadaverine(MDC) staining. The expression levels of LC3B, beclin1 and mTOR were detected by Western blot analysis. Results Compared with the control group, the number of autophagosome in the model group increased;the expression of LC3B and beclin1 significantly increased;while the expression of mTOR significantly decreased. Compared with the model group, the number of autophagosome decreased remarkably;the expression of LC3B and beclin1 severely decreased, and the expression of mTOR obviously increased in 200 or 400 μg/mL APS-treated group. Conclusion APS reduces the level of autophagy, down-regulates the expression of LC3B and beclin1, and increases mTOR expression in the autophagic model of A549 cells induced by XOD.