文献类型：期刊文献

英文题名：Gene polymorphisms (rs324957, rs324981) in NPSR1 are associated with increased risk of primary insomnia A cross-sectional study

作者：Xie, Yuping[1];Zhao, Yuan[2];Zhou, Liya[3];Zhao, Lijun[4];Wang, Jinfeng[1];Ma, Wei[1];Su, Xiaoyan[1];Hui, Peilin[1];Guo, Bin[1];Liu, Yu[1];Fan, Jie[2];Zhang, Shangli[1];Yang, Jun[2];Chen, Wenjuan[2];Wang, Jing[5]

第一作者：Xie, Yuping

通信作者：Xie, YP[1]

机构：[1]Gansu Prov Hosp, Sleep Med Ctr, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Lanzhou, Gansu, Peoples R China;[3]Gansu Prov Hosp, Electroencephalogram Room, Lanzhou, Gansu, Peoples R China;[4]Univ Adelaide, Adelaide Med Sch, Adelaide, SA, Australia;[5]Lanzhou Univ, Sch Basic Med Sci, Lanzhou, Gansu, Peoples R China

第一机构：Gansu Prov Hosp, Sleep Med Ctr, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Hosp, Sleep Med Ctr, Lanzhou 730000, Gansu, Peoples R China.

年份：2020

卷号：99

期号：34

起止页码：e21598

外文期刊名：MEDICINE

收录：;Scopus(收录号：2-s2.0-85089957866);WOS:【SCI-EXPANDED(收录号:WOS:000579455900023)】；

基金：This work was funded by the Gansu Health Industry Research Project (GSWSKY2016-07) and the National Natural Science Foundation of China (81560228).

语种：英文

外文关键词：neuropeptide S receptor; objective sleep phenotypes; polymorphism; primary insomnia

摘要：Neuropeptide S and neuropeptide S receptor (NPSR1) are associated with sleep regulation. Herein, the possible contribution of 6 polymorphisms in NPSR1 on the chromosome to primary insomnia (PI) and objective sleep phenotypes was investigated. The study included 157 patients with PI and 133 age- and sex-matched controls. All subjects were investigated by polysomnography for 3 consecutive nights. The genotyping of 6 polymorphisms was carried out by polymerase chain reaction-restriction fragment length polymorphism method. A significant difference was detected for rs324957 and rs324981 between PI and controls. The PI patients had a higher frequency of AA than controls in rs324957 (P = .02) and rs324981 (P = .04). However, for other single nucleotide polymorphisms (rs323922, rs324377, rs324396, and rs324987), no significant differences were observed between PI patients and controls. There were 2 different allelic combinations that were associated with PI susceptibility (CATGTC, GCCAAT) and its risk factor. A significant difference in sleep latency was observed among 3 genotype carriers of NPSR1 gene polymorphism rs324957 in PI group (P = .04), with carriers of the A/A genotype having the longest sleep latency (mean +/- SD: 114.80 +/- 58.27), followed by the A/G genotype (112.77 +/- 46.54) and the G/G genotype (92.12 +/- 42.72). This study provided the evidence that the NPSR1 gene polymorphisms (rs324957, rs324981) might be susceptibility loci for PI. Further studies are needed to explore the role of NPSR1 gene polymorphisms in molecular mechanisms of PI in a larger sample size.