文献类型：期刊文献

英文题名：Development and evaluation of poly adenosine 5′-diphosphate-ribose polymerase 1 immobilization-based receptor chromatography

作者：Dou, Jianwei[1];Zhu, Zhongbo[2];Li, Yan[2];Yang, Shuo[2];Guo, Zhanzi[2];Li, Kangle[1];Ren, Cuicui[3];Huang, Linhong[4];He, Jianyu[5]

第一作者：Dou, Jianwei

通信作者：He, JY[1]

机构：[1]Xi An Jiao Tong Univ, Sch Pharm, Xian, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Sch Basic Med, Lanzhou, Peoples R China;[3]First Hosp Xian, Xian, Peoples R China;[4]Honghui Hosp, Xian, Peoples R China;[5]Xi An Jiao Tong Univ, Sch Basic Med Sci, Dept Pharmacol, Hlth Sci Ctr, Xian 710061, Peoples R China

第一机构：Xi An Jiao Tong Univ, Sch Pharm, Xian, Peoples R China

通信机构：[1]corresponding author), Xi An Jiao Tong Univ, Sch Basic Med Sci, Dept Pharmacol, Hlth Sci Ctr, Xian 710061, Peoples R China.

年份：2021

卷号：44

期号：4

起止页码：793

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20205209695393);Scopus(收录号：2-s2.0-85098120254);WOS:【SCI-EXPANDED(收录号:WOS:000602661300001)】；

基金：National Natural Science Foundation of China; Grant Numbers: 81873311, 81573983, and 81674028; Science and Technology Project of Shaanxi Province; Grant Number: 2019SF-008.

语种：英文

外文关键词：affinity chromatographic model; breast cancer; poly adenosine 5′ ‐ diphosphate‐ ribose polymerase 1; traditional Chinese medicine; Yanghe decoction

摘要：Yanghe decoction is a traditional Chinese medicine prescription and has been used for breast cancer treatment for many years. However, the effective ingredients in the decoction have not been identified. The expression of poly(ADP-ribose) polymerase-1 is highly related to breast cancer. Using poly(ADP-ribose) polymerase-1 as a probe, we expressed the haloalkane dehalogenase-tagged protein in BL21(DE3) E. coli, immobilized it on hexachlorocaproic acid-modified macroporous silica gel, and established a poly(ADP-ribose) polymerase-1 chromatographic model. The feasibility of the model was verified by testing the retention behaviors of five drugs on the protein column. We applied the model in screening the bioactive components in yanghe decoction. Rutin, liquiritin, and a compound ([M-H](-) 681.7) were identified to be the potential bioactive ingredients. We studied the binding property between rutin and poly(ADP-ribose) polymerase-1 by injection amount dependent method, competitive studies, and molecular docking. We found that rutin can bind to the protein through the typical inhibitor binding site of the protein. Therefore, the chromatographic model is a useful tool to screen bioactive compounds from traditional Chinese medicine. The method is fast, reliable, and applicable to other functional proteins that can screen the potential lead compounds for the treatment of the related diseases.