文献类型：期刊文献

中文题名：“相恶”配伍黄芩生姜对湿热模型大鼠胃肠功能的影响

英文题名：Effects of Huangqin and Ginger on Gastrointestinal Function in Rat Models with Dampness-heat based on"Mutual Inhibition"

作者：虎勤[1];邓婕[2];杨志军[2];张瑞欣[2];何建桥[2];杨秀娟[2];吴溪玮[2]

第一作者：虎勤

机构：[1]兰州现代职业学院卫生健康学院,甘肃兰州730300;[2]甘肃中医药大学药学院,甘肃兰州730000

第一机构：兰州现代职业学院卫生健康学院,甘肃兰州730300

年份：2024

卷号：37

期号：5

起止页码：47

中文期刊名：西部中医药

外文期刊名：Western Journal of Traditional Chinese Medicine

收录：CSTPCD

基金：国家自然科学基金地区基金(82160755);甘肃省中医药管理局科研基金(GZK-2019-20);甘肃省教育厅高等教育教学成果培育项目(2020-120);甘肃省教育厅科技创新项目(2022A-068)。

语种：中文

中文关键词：胃肠功能;湿热型;黄芩;生姜;配伍;大鼠

外文关键词：gastrointestinal function;dampness-heat pattern;Huangqin;ginger;compatibility;rat

摘要：目的:比较黄芩与生姜配伍前后对湿热模型大鼠胃肠功能的影响,探究两药“相恶”内涵。方法:将50只SD大鼠随机分成正常组、模型组、黄芩组,不同配比黄芩生姜组(1∶1及2∶1),每组10只。采用复合因素法制备大鼠胃肠湿热模型,造模成功后连续7天给予相应药物灌胃,每日1次;取材后,苏木精-伊红染色法(hematoxylin-eosin staining,HE)观察各组大鼠胃、结肠组织病理学形态变化情况;计算大鼠胃内残留率;硝酸还原酶法检测大鼠血清一氧化氮(nitric oxide,NO)水平;酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)测定各组大鼠血清肿瘤坏死因子α(tumor necrosis factor α,TNF-α)、白细胞介素10(interleukin-10,IL-10)等炎症因子表达水平;ELISA法检测胃及结肠组织神经肽P物质(substance P,SP)、血管活性肠肽(vasoactive intestinal peptide,VIP)、生长抑素(somatostatin,SS)、胃泌素(gastrin,GAS)等胃肠激素含量。结果:干预后,与模型组比较,黄芩组、黄芩生姜1∶1组大鼠胃残留率降低(P<0.05),黄芩水煎液及黄芩生姜1∶1水煎液可下调模型大鼠血清NO、TNF-α表达水平,上调IL-10表达水平(均P<0.05),黄芩生姜1∶1水煎液可降低模型大鼠胃及结肠组织VIP、SS含量,升高SP、GAS含量(均P<0.05);与黄芩水煎液及黄芩生姜1∶1水煎液比较,黄芩生姜2∶1水煎液下调血清NO、胃及结肠组织VIP、SP水平,上调结肠组织CAS水平不明显(P>0.05)。结论:黄芩水煎液、黄芩生姜1∶1水煎液能改善湿热大鼠胃肠功能状态,主要通过调节胃肠激素、炎症因子水平发挥作用,且黄芩生姜2∶1配伍时,两药相恶减效,清热燥湿作用减弱。
Objective:To compare the effects of Huangqin(Scutellariae radix)and ginger on the gastrointestinal function of rats modeled with dampness-heat before and after compatibility,and to investigate the connotations of"mutual inhibition"between the two drugs.Methods:Fifty SD rats were randomly divided into the normal group,the model group,Huangqin group,Huangqin-ginger group with different ratios(1:1,2:1),ten rats in each group.Rat models with gastrointestinal damp-heat were established using composite factors.The corresponding drugs were given by gavage once a day for seven consecutive days after modeling;after sampling,HE was applied to observe the histopathological and morphological changes in the stomach and colon of rats in each group under the light microscope;to calculate the residual rate in the stomach;nitrate reductase method was used to detect the levels of serum NO;ELISA was adopted to detect the expressions of TNF-αand IL-10 in the serum in different groups;ELISA was used to measure the contents of SP,VIP,SS,GAS in the stomach and colon.Results:After the intervention,compared with the model group,the residual rate of the stomach decreased in Huangqin group,and Huangqin-ginger at the ratio of 1:1(P<0.05),Huangqin decoction and Huangqin-ginger decoction at the ratio of 1:1 down-regulated the levels of NO and TNF-α,and up-regulated the levels of IL-10 in rats(all P<0.05),Huangqin-ginger decoction at the ratio of 1:1 decreased the contents of VIP and SS in the stomach and colon of rats with dampness-heat and elevated the contents of SP and GAS(all P<0.05).Compared with Huangqin-ginger decoction and Huangqin-ginger decoction at the ratio of 1:1,Huangqin-ginger decoction at the ratio of 2:1 down-regulated the levels of serum NO,the levels of VIP and SP in stomach and colon,up-regulated the levels of CAS in colon insignificantly(P>0.05).Conclusion:Huangqin decoction and Huangqinginger decoction at the ratio of 1:1 can improve the state of gastrointestinal function in rats with dampness-heat,mainly through regulating the levels of gastrointestinal hormones and inflammatory factors.When Huangqin was combined with ginger at the ratio of 2:1,the two drugs inhibited each other,and the effect of clearing heat and drying dampness was weakened.