文献类型：期刊文献

中文题名：湿生扁蕾对结肠癌微环境中HMSCs恶性增殖以及CD34、CD90的影响

英文题名：Effect of Gentianopsis paludosa on malignant proliferation of HMSCs and expressions of CD34 and CD90 in the colon cancer microenvironment

作者：赵慧巧[1];卢年华[1];张旭东[1];柳娜[1];芦彦兆[1];陈正君[1];刘雪枫[1];张艳霞[1];景明[1]

第一作者：赵慧巧

机构：[1]甘肃中医药大学,兰州730000

第一机构：甘肃中医药大学

年份：2018

卷号：34

期号：3

起止页码：125

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金项目(81560717);甘肃省自然科学基金项目(1606RJZA001)

语种：中文

中文关键词：湿生扁蕾;结肠癌微环境;骨髓间充质干细胞;增殖;CD34;CD90

摘要：目的:探究湿生扁蕾乙酸乙酯部位对结肠癌微环境中人骨髓间充质干细胞(human mesenchymal stem cells,HMSCs)恶性增殖及表面标志分子CD34、CD90表达的影响。方法:通过MTT法筛选湿生扁蕾乙酸乙酯部位作用于SW480细胞和HMSCs的最佳给药剂量。建立SW480和HMSCs细胞Transwell非接触共培养模型,将试验分为正常对照组、肿瘤细胞对照组、共培养组和湿生扁蕾给药组,分别采用细胞计数试验、流式细胞术FCM观察各组细胞的增殖、周期及HMSCs表面标志分子CD34和CD90的表达情况。结果:湿生扁蕾乙酸乙酯部位最佳给药剂量为200μg/ml,100μl。与正常对照组相比,共培养组HMSCs增殖能力增强,G1期细胞比例明显降低,S期和G2/M期细胞比例明显升高,且HMSCs表面标志分子CD34阳性表达率升高,CD90阳性表达率降低。与共培养组相比,湿生扁蕾给药组HMSCs增殖能力降低,G1期细胞比例明显升高,S期和G2/M期细胞比例明显降低,且HMSCs表面标志分子CD34阳性表达率降低,CD90阳性表达率升高。结论:结肠癌微环境中HMSCs增殖能力增强,且HMSCs表面标志分子阳性表达率发生显著改变,提示HMSCs发生恶性增殖,且湿生扁蕾乙酸乙酯部位可抑制结肠癌微环境中HMSCs的恶性转变。
Objective： To investigate the effect of Gentianopsis paludosa ethyl acetate on the malignant proliferation of human bone marrow mesen- chymal stem cells and expressions of surface markers CD34 and CD90 in the colon cancer microenvironment. Methods： The optimized dose of Gentianopsis paludosa ethyl acetate acting on SW480 cells and HMSCs was screened by M33＂ assay. Transwell non-contact co-culture mod- el of SW480 and HMSCs was established. In the experiment, there were the control group, the tumor cell group, co-culture group and drug group. Cell counting test and flow cytometry were used to observe the proliferation, cycle and expression of HMSCs surface marker molecules CD34 and CD90. Results ：The optimized dose of hygropistin ethyl acetate was 200μg/ml, 100μl. Compared with the control group, the pro- liferation ability of HMSCs in co-culture group was enhanced, the proportion of cells in G1 phase was significantly decreased, cells in S phase and G2/M phase were significantly increased, and the positive expression rate of CD34 in HMSCs was reduced. Compared with the co-culture group, the proliferation ability of HMSCs was decreased, the proportion of cells in G1 phase was significantly increased, cells in S phase and G2/M phase were significantly decreased, and the expression of CD34 on HMSCs was decreased and the expression of CD90 was increased. Condueion：The proliferation of HMSCs in colon cancer micreenvirenment is enhanced and the positive expression rate of CD34 on the sur- face of HMSCs significantly changes, which indicates that HMSCs proliferate in a malignant manner. Gentianopsis paludosa Ethyl acetate in- hibits the malignant transformation of HMSCs in colon cancer mieroenvironment.