文献类型：期刊文献

中文题名：高乌头不同溶剂萃取部分对小鼠的急性毒性研究

英文题名：Study of Acute Toxicity Different Extraction Parts of Aconitum sinomontanum Nakai on Mice

作者：张立军[1];李芸[1];张艳霞[2];杨志军[1];马骏[1];戴海蓉[1]

第一作者：张立军

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2018

卷号：25

期号：3

起止页码：53

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金(81560650)

语种：中文

中文关键词：高乌头;不同溶剂萃取部分;急性毒性;半数致死量;最大给药量;小鼠

外文关键词：Aconitum sinomontanum Nakai; different extraction parts; acute toxicity; LD50; maximum dose; mice

摘要：目的比较高乌头不同溶剂萃取部分的急性毒性大小,筛选高乌头的毒性部位,为阐明高乌头的毒性成分提供依据。方法采用系统溶剂法对高乌头95%乙醇提取物进行萃取,依次得到石油醚、三氯甲烷、乙酸乙酯、正丁醇、乙醇和水提6个不同的溶剂萃取部分,通过半数致死量(LD50)和最大给药量法对高乌头不同溶剂萃取部分的急性毒性进行比较研究。结果高乌头三氯甲烷、水提及正丁醇部分LD50分别为89.65、1805.40、24 409.41 mg/kg,95%可信区间分别为76.39~108.41、1521.60~2240.00、20 422.54~24 246.95;高乌头石油醚、乙酸乙酯及乙醇部分最大给药量分别为2686.01、3108.13、28 376.21 mg/kg。结论高乌头三氯甲烷部分毒性最大,乙醇部分毒性最小。
Objective To compare the toxicity of different extraction parts of Aconitum sinomontanum Nakai; To screen out “toxic sections” of Aconitum sinomontanum Nakai; To provide references for further study on toxicity components of Aconitum sinomontanum Nakai. Methods Systematic solvent method was used to extract the 95% ethanol extracts of Aconitum sinomontanum Nakai, and six different extraction fractions （petroleum ether, chloroform, ethyl acetate, butanol, alcohol and water） were obtained. Median lethal dose （LD50） and maximum dose method were used to conduct comparative study on acute toxicity of different extraction parts of Aconitum sinomontanum Nakai. Results Chloroform, water and butanol extractions in LD50 of Aconitum sinomontanum Nakai were 89.65, 1805.40 and 24 409.41 mg/kg, and 95% confidence limits were 76.39～108.41, 1521.60～2240.00 and 20 422.54～24 246.95, respectively. The maximum dose of petroleum ether, ethyl acetate and alcohol extractions were 2686.01, 3108.13 and 28 376.21 mg/kg, respectively. Conclusion The maximum toxicity is the extracted section of chloroform, and the minimal toxicity is the extracted section of ethanol.