文献类型：期刊文献

英文题名：Advances in Cytosolic Delivery of Proteins: Approaches, Challenges, and Emerging Technologies

作者：Zhang, Wenyan[1];Liu, Huiling[2];Zhu, Bingdong[3];Li, Wen[2];Han, Xue[2];Fu, Jiaojiao[1];Luo, Renjie[1];Wang, Haiyan[1];Wang, Jinxia[1]

第一作者：张文奕

通信作者：Liu, HL[1]

机构：[1]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou, Gansu, Peoples R China;[2]Gansu Prov Hosp, Lanzhou, Gansu, Peoples R China;[3]Lanzhou Univ, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Prov Hosp, Lanzhou, Gansu, Peoples R China.

年份：2025

外文期刊名：CHEMISTRY & BIODIVERSITY

收录：;Scopus(收录号：2-s2.0-85218039072);WOS:【SCI-EXPANDED(收录号:WOS:001422738300001)】；

基金：The authors thank the National Natural Science Foundation of China (82260557), Lanzhou Science and Technology Plan Project (2023-ZD-21), and Gansu Provincial People's Hospital Fund (22GSSYD-39) for financial support.

语种：英文

外文关键词：nanocarriers; protein chemical modification; protein delivery

摘要：Although therapeutic proteins have achieved recognized clinical success, they are inherently membrane impermeable, which limits them to acting only on extracellular or membrane-associated targets. Developing an efficient protein delivery method will provide a unique opportunity for intracellular target-related therapeutic proteins. In this review article, we summarize the different pathways by which cells take up proteins. These pathways fall into two main categories: One in which proteins are transported directly across the cell membrane and the other through endocytosis. At the same time, important features to ensure successful delivery through these pathways are highlighted. We then provide a comprehensive overview of the latest developments in the transduction of covalent protein modifications, such as coupling cell-penetrating motifs and supercharging, as well as the use of nanocarriers to mediate protein transport, such as liposomes, polymers, and inorganic nanoparticles. Finally, we emphasize the existing challenges of cytoplasmic protein delivery and provide an outlook for future progress.