文献类型：期刊文献

中文题名：痛泻二草方对肝郁脾虚型溃疡性结肠炎大鼠相关基因的影响

英文题名：Effects of the Tongxie Ercao decoction on the expression of related genes of ulcerative colitis rats of liver-depression and spleen-deficiency tpye

作者：许雅清[1];李海龙[1];邱家权[1];段永强[1];程小丽[1];吴玉泓[1]

第一作者：许雅清

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2016

卷号：8

期号：8

起止页码：3

中文期刊名：中医临床研究

外文期刊名：Clinical Journal of Chinese Medicine

基金：研究生创新基金项目(CX2014-04)

语种：中文

中文关键词：溃疡性结肠炎;Mapk8基因;Fas基因;痛泻二草方

外文关键词：Ulcerative colitis; Mapk8 gene; Fas gene; The Tongxie Ercao decoction

摘要：目的:探讨痛泻二草方对肝郁脾虚型溃疡性结肠炎模型大鼠Mapk8、Fas基因表达的影响。方法:采用TNBS(2,4,6-三硝基苯磺酸)+乙醇灌肠及束缚法建立肝郁脾虚型UC动物模型。将90只大鼠随机分为空白组、模型组、痛泻二草方高剂量、中剂量、低剂量治疗组及阳性对照组,经药物干预后采用RT-q PCR法检测各组大鼠Mapk8、Fas基因的表达。结果:与空白组比较,模型组结肠组织内Mapk8、Fas基因表达水平均增高,差异显著(P<0.01);与模型组比较,各治疗组大鼠结肠组织内Mapk8、Fas的基因表达水平都有不同程度的下降(P<0.01或P<0.05),尤以高剂量治疗组差异最显著(P<0.01)。结论:痛泻二草方能够下调Mapk8、Fas基因的表达水平,进而减轻黏膜炎症反应,起到修复黏膜的作用。
Objective To study effects of the Tongxie Ercao decoction on the expression of Mapk8 and Fas genes of ulcerativecolitis rats. Methods： The rat model with liver-depression and spleen-deficiency syndrome of UC was established by using enema of TNBS（2,4,6-three trinitrobenzene sulfonic acid） and ethanol under restraint stress, and 90 rats were divided into 6 groups randomly, which werethe blank group, the model group, the TXECD group of high dosage, the TXECD group of medium dosage, the TXECD group of lowdosage and the SASP group. RT-qPCR was applied to measure the expression of Mapk8 and Fas genes after drug intervention. Results： Thegenes expressions of Mapk8 and Fas in the model group were much higher than those in the blank group （P〈0.01 or P〈0.05）. Comparedwith the model group, the genes expressions of Mapk8 and Fas in all treatment groups were decreased （P〈0.01 or P〈0.05）, and the TXECDgroup of high dosage showed most curative effects （P〈0.01 or P〈0.05）. Conclusion： The effects of TXECD were associated with downregulation of the expression levels of Mapk8 and Fas, and reduction of mucosal inflammation and repairment of mucous membrane.