文献类型：期刊文献

中文题名：连作当归叶际、根际土细菌多样性及不同病害防控技术

英文题名：Bacterial Diversity in Phyllosphere and Rhizosphere Soil of Angelica sinensis in Continuous Cropping and Effects of Different Disease Control Measures

作者：唐湛文[1,2];杨涛[1,3];彭桐[3];王引权[3];赵疆[1];沙洁[1];王治业[1]

第一作者：唐湛文

机构：[1]甘肃省科学院生物研究所,甘肃省微生物资源开发利用重点实验室,兰州730000;[2]重庆市药物种植研究所,重庆市道地药材规范化生产工程技术研究中心,重庆408435;[3]甘肃中医药大学药学院,兰州730000

第一机构：甘肃省科学院生物研究所,甘肃省微生物资源开发利用重点实验室,兰州730000

年份：2025

卷号：31

期号：15

起止页码：199

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：;北大核心:【北大核心2023】；

基金：甘肃省知识产权应用项目(22ZSCQ037);甘肃省科学院重点研发项目(2023ZDYF-01);甘肃省科学院产业化项目(2024YLCY-03);兰州市青年科技人才创新项目(2023-QN-150)。

语种：中文

中文关键词：当归;16S高通量测序;叶际根际;多黏类芽孢杆菌;枯草芽孢杆菌

外文关键词：Angelica sinensis;16S high-throughput sequencing;phyllosphere and rhizosphere;Paenibacillus polymyxa;Bacillus subtilis

摘要：目的:研究当归叶际和根际土细菌多样性及叶面喷施复合菌剂、水杨酸、冠菌素对细菌多样性及病害率、产量的影响,以期为功能性极简群落的人工构建及通过叶面处理调控根际提供理论依据和指导。方法:在大田连作条件下,叶面喷施复配菌剂、水杨酸、冠菌素、无菌水,常规方法称量百株重,统计病害发生率;借助16S高通量测序技术分析当归叶际、根际土微生物组成、多样性、组间差异,预测其功能。结果:与空白组比较,喷施水杨酸、冠菌素均导致当归的产量、根腐病发生率明显降低(P<0.05);水杨酸处理组阿魏酸含量降低,藁本内酯升高(P<0.05);冠菌素处理组,两者都升高。叶际和根际土微生物群落及功能差异显著,叶际微生物多样性低,全部为革兰氏阴性菌,主要分布在蓝藻菌门和变形菌门,功能单调;根际土微生物多样性高,主要分布在变形菌门、放线菌门、酸杆菌门、拟杆菌门等,功能丰富。不同处理叶喷均能调控根际土微生物,而且对根际土的影响明显大于叶际。冠菌素处理组根际土变形菌门丰度明显降低,降低了根际土硝酸还原微生物,芳香族化合物降解微生物丰度,影响养分循环和自毒物质降解,导致减产。冠菌素较水杨酸处理,根际土芽孢杆菌属、链霉菌属丰度明显提高,提升了病害防治效果。结论:叶片喷施冠菌素和水杨酸,可以明显调控根际土细菌群落组成与功能,降低产量和病害发生率。
Objective:To investigate the bacterial diversity in the rhizosphere soil and phyllosphere of Angelica sinensis and examine the effects of foliar applications of a composite bacterial agent,salicylic acid,and coronatine on the bacterial diversity,disease incidence,and plant yield,thus providing a theoretical basis and guidance for the artificial construction of functional minimal communities and the regulation of rhizosphere through foliar treatments.Methods:Under continuous cropping conditions in the field,foliar applications of a composite bacterial agent,salicylic acid,coronatine,and sterile water were conducted.The 100-plant weight was measured via the conventional method,and the incidence of diseases was recorded.The microbial community composition,diversity,and inter-group differences in the phyllosphere and rhizosphere soil of A.sinensis were analyzed by 16S highthroughput sequencing,and the potential microbial functions were predicted.Results:Compared with the blank control,foliar applications of salicylic acid and coronatine both significantly reduced the yield and root rot incidence of A.sinensis.The foliar application of salicylic acid decreased the content of ferulic acid and increased that of ligustilide.The foliar application of coronatine increased the content of both ferulic acid and ligustilide.The microbial communities and functions in the phyllosphere and rhizosphere soil were significantly different.The phyllosphere had lower microbial diversity,with all bacteria being Gram-negative,mainly Cyanobacteria and Proteobacteria with limited functions.The rhizosphere soil had higher microbial diversity,harboring dominant phyla including Proteobacteria,Actinobacteria,Acidobacteria,and Bacteroidetes with rich functions.All foliar treatments regulated the microbial community in the rhizosphere soil,with a more significant effect on the microbial community in the rhizosphere soil than that in the phyllosphere.The coronatine treatment significantly reduced the abundance of Proteobacteria and nitrate-reducing and aromatic compound-degrading microorganisms in the rhizosphere soil,thus affecting nutrient cycling and autotoxic substance degradation and leading to a yield reduction.Compared with the salicylic acid treatment,the coronatine treatment significantly increased the abundance of Bacillus and Streptomyces in the rhizosphere soil,demonstrating enhanced disease control efficacy.Conclusion:Foliar application of coronatine and salicylic acid can significantly regulate the composition and function of bacterial communities in the rhizosphere soil,thereby reducing the disease incidence and the plant yield.