文献类型：期刊文献

英文题名：Huanglian decoction prevents and treats radiation-induced intestinal injury in lung cancer by regulating endoplasmic reticulum stress

作者：Shi, Liang-liang[1,2,3];Chen, Ya-ping[1,2];Guo, Hao-ming[1,2];Wang, Xue-peng[1,2];Li, Yang-yang[1,2];Wang, Shuai-zhe[1,2];Zhang, Shang-zu[1,2];Zhuo, Qi-hong[1,2];Liu, Yong-qi[1,2];Wei, Ben-jun[1,2];Zhang, Li-ying[1,4]

第一作者：Shi, Liang-liang

通信作者：Wei, BJ[1];Zhang, LY[1];Wei, BJ[2];Zhang, LY[3]

机构：[1]Gansu Univ Tradit Chinese Med, Lanzhou, Peoples R China;[2]Key Lab Dunhuang Med & Transformat Prov & Minister, Lanzhou, Peoples R China;[3]Jiugang Hosp, Jiayuguan, Peoples R China;[4]Key Lab Tradit Chinese Med Explorat & Innovat Tran, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Lanzhou, Peoples R China;[2]corresponding author), Key Lab Dunhuang Med & Transformat Prov & Minister, Lanzhou, Peoples R China;[3]corresponding author), Key Lab Tradit Chinese Med Explorat & Innovat Tran, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

外文期刊名：JOURNAL OF NATURAL MEDICINES

收录：;Scopus(收录号：2-s2.0-85211434558);WOS:【SCI-EXPANDED(收录号:WOS:001370728400001)】；

基金：This study was funded by the National Natural Science Foundation of China (82260882) and the Gansu Provincial Department of Education (2022B-116).

语种：英文

外文关键词：Huanglian decoction; Lung cancer; Radiotherapy; Intestinal injury; Endoplasmic reticulum stress

摘要：Radiotherapy is a primary treatment method for lung cancer, and radiation-induced lung injury has been widely studied. However, the lung and intestines are closely related, and patients receiving lung radiotherapy often experience gastrointestinal reactions. Huanglian Decoction has proven effective in treating intestinal diseases, but its role in radiation-induced intestinal injury in lung cancer has not yet been demonstrated. The study investigated the potential protective mechanisms of Huanglian Decoction against radiation-induced intestinal injury in lung cancer. In vivo experiments were conducted to examine the morphological changes. Changes in endoplasmic reticulum stress-related proteins were assessed using electron microscopy, immunohistochemistry, immunofluorescence, and Western blotting. In vitro studies involved the overexpression of TRPA1 protein in NCM460 cells via lentiviral vectors. Tumor-bearing mice exhibited severe damage to both lung and colon tissues following radiotherapy, with elevated levels of IL-33, increased expression of ST2L and TRPA1 in colon tissues, higher levels of endoplasmic reticulum stress-related proteins, and the presence of apoptosis and inflammatory responses. Huanglian Decoction reduced radiation-induced intestinal injury by lowering IL-33 levels, which in turn reduced endoplasmic reticulum stress response in colon tissues. In TRPA1-overexpressing NCM460 cells, Huanglian Decoction decreased TRPA1 expression levels and significantly alleviated endoplasmic reticulum stress response. Conclusively, the results indicate that Huanglian Decoction alleviates radiation-induced intestinal endoplasmic reticulum stress by reducing IL-33 levels, subsequently inhibiting the expression of ST2L and TRPA1 in colon tissues. This demonstrates the protective effect of Huanglian Decoction on the intestines during lung cancer radiotherapy, highlighting its promising clinical application in radiation protection.