文献类型：期刊文献

英文题名：Molecular Spectroscopy Evidence of Effective Constituents in the Binding of Angelica sinensis to Bovine Serum Albumin: Comparative Binding and Thermodynamic Profile

作者：Wang, Zhenzhong[1,2,3]; Deng, Xuehua[1,2,3]; Hou, Jia[4]; Fan, Jing[1,2,3]

第一作者：Wang, Zhenzhong

机构：[1] Pharmacy College of Hubei University of Medicine, Shiyan, 420000, China; [2] Key Lab of Wudang Characteristic Herbal Study in Hubei Province, Shiyan, 420000, China; [3] Engineering Research Center for Herbal Utilization of Hubei Province, Shiyan, 420000, China; [4] Gansu University of Chinese Medicine, Lanzhou, 730000, China

第一机构：Pharmacy College of Hubei University of Medicine, Shiyan, 420000, China

通信机构：[1]Pharmacy College of Hubei University of Medicine, Shiyan, 420000, China

年份：2019

卷号：85

期号：6

起止页码：1064

外文期刊名：Journal of Applied Spectroscopy

收录：EI(收录号：20242616356814);Scopus(收录号：2-s2.0-85061061848)

语种：英文

外文关键词：Blood - Fluorescence spectroscopy - Mammals - Molecular spectroscopy - Organic acids - Spectroscopic analysis - Surface plasmon resonance

摘要：Some bio-effective constituents, such as organic acids and phthalides, from the root of Angelica sinensis can be observed in the blood after oral intake. In this work, two organic acids, ferulic acid and anisic acid, and two phthalides, senkyunolide I and butylidenephthalide, were selected to investigate the interaction with bovine serum albumin systematically, using a fluorescence spectroscopic method under physiological conditions. The Stern–Volmer quenching constant, binding constant, and the corresponding thermodynamic parameters and binding numbers were measured. Synchronous fluorescence and three-dimensional fluorescence spectroscopy were also used. The results provided molecular spectroscopy evidence that some constituents of Angelica sinensis are absorbed and transported into the blood. ? 2019, Springer Science+Business Media, LLC, part of Springer Nature.