文献类型：期刊文献

英文题名：Huangqi Baihe Granules Attenuate Hypobaric Hypoxia-Induced Brain Injury via HIF-1a/p53/ Caspase-3 Pathway

作者：Zhang, Xinjue[1,2];Cao, Wangjie[1,2];Pan, Mingyue[1,2];Huo, Jiawei[1,2];Zhang, Nengxian[1,2];Guo, Jiawang[1,2];Huang, Yong[1,2];Liu, Yongqi[1,2];Gong, Hongxia[1,2];Su, Yun[1,2]

第一作者：张香菊;张小娟;张晓洁;张小江;张学军;张秀娟;张秀景

通信作者：Su, Y[1]

机构：[1]Gansu Univ Chinese Med, Gansu Univ, Key Lab Mol Med & Chinese Med Prevent & Treatment, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Minist Educ, Key Lab Dunhuang Med, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Sch Basic Med Sci, Lanzhou 730050, Peoples R China.|[107351d2d02a88e1f325f]甘肃中医药大学基础医学院（敦煌医学研究所）;[10735]甘肃中医药大学;

年份：2025

卷号：19

外文期刊名：DRUG DESIGN DEVELOPMENT AND THERAPY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001599049400001)】；

基金：Natural Science Foundation of Gansu Province (23JRRA1210) ; Lanzhou Science and Technology Program Fund Grant (2022-2-106) ; Science and Technology Innovation Fund Grant for Higher Education Institutions of Gansu Provincial Department of Education (2023A-087) ; Scientific Research Start-up Fund Grant for Introduced Talents of Gansu University of Traditional Chinese Medicine (2023YJRC-06) .

语种：英文

外文关键词：Huangqi Baihe Granule; hypobaric hypoxia; high altitude cerebral edema; HIF-1 alpha/p53/caspase-3; apoptosis

摘要：Background: High altitude cerebral edema (HACE), a severe central nervous system dysfunction caused by acute plateau hypoxia, involves oxidative stress and apoptosis. Huangqi Baihe granules (HQBHG) show efficacy against these processes, but their mechanism remains unclear. Purpose: This study evaluated Huangqi Baihe granules (HQBHG)'s efficacy in treating High altitude cerebral edema (HACE) and elucidated its mechanism. Methods: UPLC-MS/MS characterized Huangqi Baihe granules (HQBHG)'s chemical composition. Seventy-two SD rats were kg), and HQBHG low/medium/high-dose groups (1.105 g/kg d, 2.21 g/kg d, 4.42 g/kg d). Except NC, all underwent 72-hour staining), oxidative stress markers (Reactive oxygen species, Superoxide dismutase), and histopathology (HE/Nissl staining) were assessed. Network pharmacology (TCMSP, GenGards, OMIM, Drugbank) and transcriptomics identified Huangqi Baihe granules (HQBHG) targets and pathways. Apoptosis signaling (HIF-1 alpha/p53/Caspase-3) was validated via immunofluorescence, TUNEL, Transmission Electron Microscope, Western Blotting, and qRT-PCR. Results: Hypobaric hypoxia caused brain injury and blood-brain barrier disruption. Network and transcriptome analyses linked Huangqi Baihe granules (HQBHG)'s effects to HIF-1 alpha/p53/Caspase-3 pathway, involving key genes. Huangqi Baihe granules (HQBHG) intervention attenuated brain injury, oxidative stress, and apoptosis, suppressing HIF-1 alpha/p53/Caspase-3 pathway activation. Conclusion: We demonstrated for the first time that Huangqi Baihe granules (HQBHG) may reduce brain tissue injury by regulating the HIF-1 alpha/p53/Caspase-3 signaling pathway, ameliorating blood-brain barrier disruption induced by low-pressure hypoxia, imbalance of oxidative stress in the brain tissues, and inhibiting apoptosis in the brain cells.