文献类型：期刊文献

中文题名：毒性中药高乌头炮制前后水煎液与三氯甲烷部分对小鼠的镇痛抗炎作用

英文题名：Effects of water decoction and chloroform extraction fraction before and after processing of Aconitum sinomontanum Nakaion on analgesic and anti-inflammatory in mice

作者：张立军[1];李芸[1];杨志军[1];马骏[1];张艳霞[2]

第一作者：张立军

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃中医药大学甘肃省中药药理与毒理学重点实验室,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2017

卷号：33

期号：7

起止页码：624

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目(81560650)

语种：中文

中文关键词：高乌头;制高乌头;水煎液;三氯甲烷部分;镇痛;抗炎

外文关键词：Aconitum Sinomontanum Nakai; processed Aconitum Sinomontanum Nakai; water decoction; chloroform extraction fraction; analgesic activity; anti-inflammatory activity

摘要：目的研究高乌头炮制前后水煎液和三氯甲烷部分对小鼠镇痛抗炎作用。方法取SPF级昆明小鼠,按照体重随机分为10组:空白对照组(0.5%甲基纤维素)、阳性对照组(消炎痛10 mg·kg^(-1),氢溴酸高乌甲素1.6 mg·kg^(-1))、高乌头水煎液高低2个剂量组(0.9,0.3g·kg^(-1)),制高乌头水煎液高低2个剂量组(0.9,0.3 g·kg^(-1)),高乌头三氯甲烷高低2个剂量组(4.5,1.5 mg·kg^(-1)),制高乌头三氯甲烷高低2个剂量组(4.5,1.5 mg·kg^(-1)),每组10只。采用热板法对高乌头炮制前后水煎液及三氯甲烷部分进行镇痛作用评价;用二甲苯致鼠耳肿胀实验对其进行抗炎作用评价。结果镇痛作用:在热板法实验中,给药后30 min,制品水煎液组小鼠痛阈值为(24.1±6.3)s;给药后120 min后,制品水煎液组痛阈值为(49.7±9.7)s,生品三氯甲烷部分高低2个剂量组、制品三氯甲烷部分高剂量组痛阈值分别为(49.8±7.4),(47.3±6.3),(45.1±7.1)s,与空白对照组相比,差异具有统计学意义(均P<0.05),说明高乌头的镇痛作用发生在给药后120 min左右。抗炎作用:高乌头生品水煎液组、制品水煎液高低2个剂量组、高乌头生品和制品三氯甲烷部分高低2个剂量组小鼠耳肿胀度分别为(16.9±11.2),(15.2±4.8),(17.7±6.4),(14.2±4.2),(16.0±4.5),(15.7±5.2),(12.5±5.5)mg,与空白对照组相比,差异具有统计学意义(均P<0.01)。制品各组与相应生品各组比较,差异无统计学意义(P>0.05)。结论乌头生品具有很强的镇痛抗炎活性,炮制后其镇痛抗炎活性仍然保持,并有增强的趋势。
Objective To study the effects of water decoction and chloroform extraction fraction before and after processing of Aconitum sinomontanum Nakai（ASN） on the analgesic and anti-inflammatoryin mice.Methods Kunming mice were randomly divided into 10 groups（10 mice in each group） according to the weight：blank control group（0.5% methylcellulose）,positive control group（10 mg · kg^-1indomethacin ± 1.6 mg · kg^-1 lappaconitine）,the high and low dose groups of water decoction of raw ASN（0.9,0.3 g · kg^-1）,the high and low dose groups of water decoction processed product（0.9,0.3g · kg^-1）,the high and low dose groups of chloroform extraction fraction of ASN（4.5,1.5 mg·kg^-1）,the high and low dose groups of chloroform extraction fraction of processed product（4.5,1.5 mg·kg^-1）.Hot plate was used to study the analgesic effect of water decoction and chloroform extraction fraction before and after processing of ASN.Auricular swelling induced by dimethylbenzene in mice were observed for the evaluation of anti-inflammatory effects of it.Results Analgesic activity：Inthe hot plate test,the pain threshold of high dose groups of water decoction of processed product at 30 minafter the last administration was（24.1 ± 6.3） s,the pain threshold of high dose groups of water decoction of processed product,high and low dose groups of chloroform extraction fraction of raw product,high dose groups of chloroform extraction fraction of processed product at 120 min after the last administration were（49.7 ±9.7）,（49.8 ±7.4）,（47.3 ± 6.3）,（45.1 ± 7.1） s,compared with blank control group,the difference were significant（all P 0.05）,respectively.Anti-inflammatory activity：The swelling degree of high dose groups of water decoction of raw product,high and low dose groups of water decoction processed product,high and low dose groups of chloroform extraction fraction of raw and processed product were（16.9 ± 11.2）,（15.2 ± 4.8）,（17.7 ± 6.4）,（14.2 ± 4.2）,（16.0 ±4.5）,（15.7 ±5.2）,（12.5 ±5.5） mg,compared with blank control group,the difference were significant（all P 0.01）.The each groupof processed products compared with the each group of raw product,the difference was not significant（P 0.05）.Conclusion The raw Aconitum sinomontanum Nakai have strong analgesic and anti-inflammatory effects,and processed product still remain strong analgesic and anti-inflammatory effects,and have a trend to increase compared with the raw product.