文献类型：期刊文献

中文题名：基于NLRP3炎症小体探讨当归红芪超滤物对放射性心肌细胞损伤的保护作用

英文题名：Protective effects of Angelicae and Astragalus ultra-filtration on radiation-induced myocardial cell injury based on NLRP3 inflammasome

作者：李淑玲[1];张晓刚[2];王旭勇[2];徐晓东[1]

第一作者：李淑玲

机构：[1]甘肃中医药大学附属医院心血管中心,甘肃兰州730020;[2]甘肃中医药大学中西医结合学院,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2025

卷号：41

期号：7

起止页码：932

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：;北大核心:【北大核心2023】；

基金：甘肃省自然科学基金资助项目(21JR11RA150)。

语种：中文

中文关键词：当归红芪超滤物;心肌细胞焦亡;NOD样受体热蛋白结构域相关蛋白3

外文关键词：Angelicae and Astragalus ultra-filtration;cardiomyocyte pyroptosis;NOD-like receptor thermal protein domain-associated protein 3 inflammasome

摘要：目的基于NOD样受体热蛋白结构域相关蛋白3(NLRP3)炎症小体探讨当归红芪超滤物(AAU)对X线辐射致H9C2心肌细胞损伤的保护作用。方法将H9C2心肌细胞分为空白组、模型组和低、中、高剂量实验组。模型组和低、中、高剂量实验组用X射线6 Gy照射构建H9C2心肌细胞损伤模型。建模成功后,模型组和空白组均给予0.9%NaCl处置,低、中、高剂量实验组分别给予2%、5%、10%AAU含药血清0.5 mL进行培养。培养24 h后,用EdU染色法观察细胞存活率,用蛋白质印迹法检测NLRP3、焦亡相关斑点样蛋白(ASC)、胱天蛋白酶-1(Caspase-1)等蛋白的表达水平。结果中、高剂量实验组和模型组、空白组的细胞存活率分别为(89.91±1.16)%、(91.66±1.93)%、(78.61±1.30)%和(98.10±0.20)%,NLRP3蛋白相对表达水平分别为0.79±0.05、0.74±0.04、0.94±0.02和0.53±0.12,ASC蛋白相对表达水平分别为0.40±0.04、0.41±0.07、0.48±0.01和0.22±0.02,Caspase-1蛋白相对表达水平分别为0.25±0.03、0.23±0.01、0.58±0.04和0.18±0.01,IL-18蛋白相对表达水平分别为0.67±0.03、0.62±0.02、0.91±0.07和0.61±0.03,IL-1β蛋白相对表达水平分别为0.41±0.06、0.38±0.02、0.78±0.02和0.34±0.09。模型组的上述指标与中、高剂量实验组相比,在统计学上差异均有统计学意义(均P<0.05)。结论AAU可能通过调节NLRP3炎症小体对X线辐射导致的 H_(9)C_(2)心肌细胞损伤具有保护作用。
Objective To investigate the protective effects of Angelicae and Astragalus ultra-filtration(AAU)on H,C,myocardial cell injury induced by X-ray radiation based on NOD-like receptor thermal protein domain-associated protein 3(NLRP3)inflammasome.Methods The H,C,myocardial cells were divided into blank group,model group and experimental-L,-M,-H groups.The H,C,myocardial cells injury model of model group and experimental-L,-M,-H groups were constructed with X-ray 6 Gy irradiation.After successful modeling,the blank and model groups were treated with 0.9%NaCl;the experimental-L,-M,-H groups were treated with 2%,5%,10%AAU drug-containing serum 0.5 mL,respectively.After 24 h of treatment,EdU staining was used to observe cell survival rate,Western blot was used to detect the expression levels of NLRP3,apoptosis-associated speck-like protein containing a CARD(ASC)and Caspase-1 protein.Results The cell viability rates of the experimental-M,-H groups,model group and blank group were(89.91±1.16)%,(91.66±1.93)%,(78.61±1.30)%and(98.10±0.20)%;the relative expression levels of NLRP3 protein were 0.79±0.05,0.74±0.04,0.94±0.02 and 0.53±0.12;the relative expression levels of ASC protein were 0.40±0.04,0.41±0.07,0.48±0.01 and 0.22±0.02;the relative expression levels of Caspase-1 protein were 0.25±0.03,0.23±0.01,0.58±0.04 and 0.18±0.01;the relative expression levels of IL-18 protein were 0.67±0.03,0.62±0.02,0.91±0.07 and 0.61±0.03;the relative expression levels of IL-1βprotein were 0.41±0.06,0.38±0.02,0.78±0.02 and 0.34±0.09,respectively.Compared with the experimental-M,-H groups,the differences of above indicators in the model group were statistically significant(all P<0.05).ConclusionAAUmay protect H_(9)C_(2) myocardial cells from injury induced by X-ray radiation by regulating NLRP3 inflammasome.