文献类型：期刊文献

中文题名：中心组合设计-响应面法优化枸杞子中有效成分的提取工艺研究

英文题名：Optimization of the extraction process of main effective components in Lycii Fructus by central composite design-response surface method

作者：崔治家[1,2];夏鹏飞[2];杨毛毛[1];张启立[1,2];刘峰林[1,2];马肖[3];晋玲[1,2];马毅[1,2];王振恒[1,2];赵文龙[1]

第一作者：崔治家

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃省高校中(藏)药化学与质量研究省级重点实验室,兰州730000;[3]甘肃省第二人民医院,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2019

卷号：46

期号：5

起止页码：375

中文期刊名：国际药学研究杂志

外文期刊名：Journal of International Pharmaceutical Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；

基金：甘肃省自然科学基金项目(148RJZA059);中央财政引导地方科技创新平台项目(2016-A-02);甘肃省科技计划基础研究创新群体(1606RJIA323);中医药行业科研专项(201207002)

语种：中文

中文关键词：枸杞子;中心组合设计;响应面法;提取工艺

外文关键词：Lycii Fructus;central composite design;response surface method;extraction process

摘要：目的采用中心组合设计(CCD)-响应面法(RSM)优化枸杞子中有效成分的提取工艺。方法以枸杞子中主要有效成分多糖、总黄酮、芦丁和绿原酸及浸膏得率为考察指标,对提取溶剂、提取时间、料液比及提取温度等因素进行考察,并采用单因素实验确定各因素的取值范围,析因设计实验筛选对枸杞子有效成分提取效率有显著影响的因素,最速上升实验确定因素的中心值,最终采用CCD-RSM优选最佳提取工艺,并进行验证分析。结果优选出的最佳提取工艺为:室温下,以60%乙醇溶液做为提取溶剂,超声提取时间40.5 min,料液比为1∶27。结论本实验筛选出的最佳提取工艺能有效提高枸杞子中有效成分的提取率,且建立的模型预测性及重复性良好。该工艺为枸杞子的深入开发研究提供实验基础和理论依据。
Objective To optimize the extraction process of effective components in Lycii Fructus using central composite design(CCD)-response surface method(RSM).Methods The yield of main effective components,polysac-charides,flavonoids,rutin and chlorogenic acid as well as the yield of total extract were used as indicator for evaluating the effect of extraction solvent,extraction time,solid-liquid ratio,extraction temperature and other factors.The single factor experiment was conducted to determine the range of various factor parameters,the factor design experiment was performed for the screening of the factors that had significant influence on the extraction efficiency of effective components,the maximum velocity rise experiment was carried out to determine the central value of the factors,and finally the optimal extraction process was optimized by the central composite design-response surface methodology.Results The optimum extraction conditions of Lycii Fructus were obtained as follows:the extraction with 60%aqueous ethanol solution by ultrasound sonication at room temperature for 40.5 minutes,and the solid-liquid ratio was 1∶27.Conclusion The present optimal extraction process could effectively improve the extraction efficacy of effective components from Lycii Fructus,and the created CCD-RSM model and related conditions have good predictability and repeatability,which pro?vides experimental and theoretical reference for further development and research of Lycii Fructus.