文献类型：期刊文献

中文题名：当归-红芪超滤膜提取物对老龄大鼠记忆功能和神经递质的影响

英文题名：Effects of Ultra-filtration Extract from Angelica Sinensis Radix and Hedysari Radix on the Old Rats' Memory Function and Brain Neurotransmitters

作者：刘萍萍[1];李应东[1,2];周倩倩[1];孙少伯[1];刘凯[1,2]

第一作者：刘萍萍

机构：[1]甘肃中医学院,兰州730000;[2]甘肃中医学院附属医院,兰州730020

第一机构：甘肃中医药大学

年份：2013

卷号：19

期号：24

起止页码：189

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2011】；

基金：国家教育部"春晖计划"(Z2004-1-62042)

语种：中文

中文关键词：当归红芪超滤物;记忆功能;神经递质

外文关键词：ultra-filtration extract from Angelica Sinensis Radix and Hedysari Radix; memory ability;brain neurotransmitters

摘要：目的:探讨不同相对分子质量当归-红芪(当归∶红芪1∶5)超滤膜提取物对老龄大鼠记忆功能和神经递质的影响。方法:本实验选3月龄Wistar大鼠12只作为青年对照组(A组),24月龄Wistar大鼠48只随机分为老年空白对照组(B组)、当归-红芪超滤膜提取物相对分子质量5万Da(C组)、相对分子质量10万Da(D组)、20万Da组(E组),用药组分别给予相对分子质量为5万Da(1.87 g·kg-1·d-1)、10万Da(2.14 g·kg-1·d-1)、20万Da(2.47 g·kg-1·d-1)的当归-红芪超滤膜提取物,其余2组给予同体积生理盐水,连续灌胃70 d,观察各组大鼠学习记忆功能,显微镜下海马,大脑皮质组织形态改变,检测脑组织中枢神经递质乙酰胆碱(Ach),单胺类神经递质5羟色胺(5-HT),多巴胺(DA),去甲肾上腺素(NE)含量。结果:老年组大鼠与青年组大鼠相比学习能力下降;海马,大脑皮质组织形态改变存在差异,神经递质Ach,DA,NE,5-HT含量显著降低(P<0.05);与衰老组大鼠相比,当归红芪超滤膜提取物组大鼠脑组织中Ach,DA,NE,5-HT含量增高,有显著差异(P<0.05);与10万Da相对分子质量组相比,5万Da,20万Da相对分子质量组大鼠脑组织神经递质含量的影响不及10万Da相对分子质量组明显(P<0.05)。结论:当归红芪超滤膜提取物可以通过提高神经递质Ach,DA,NE,5-HT含量来改善老龄大鼠记忆功能,尤以10万Da相对分子质量组为优。
Objective： In order to investigate the effect of ultra-filtration extract from Angelica Sinensis Radix and Hedysari Radix（Angelica Sinensis Radix：Hedysari Radix 1：5） on the memory ability and brain neurotransmitters in old rats. Method： 12 healthy male Wistar rats with 3-months old were selected for the youth control group （A）, and 48 natural aging male Wistar rats with 24-months old were selected and randomly divided into four groups：the old control group （B）, 5×104 Da molecular of UFE-AH group （C）, 1×105 Da molecular of UFE-AH group （D）, and 2×105 Da molecular of UFE-AH group （E）;Group A,B were given normal saline （7 mL·kg-1·d-1） and Group C,D,E were respectively given with 5×104 Da（1.87 g·kg-1·d-1）, 1×105（2.14 g·kg-1·d-1）,2×105 Da（2.47 g·kg-1·d-1）molecular of UFE-AH by gavage. After 10 weeks, the learning and memory ability were measured, and heir brain tissue was observed （hippocampus and cortex） by microscope,the contents of the central neurotransmitter acetylcholine（Ach）, the monoamine neurotransmitter 5-hydroxytryptamine（5-HT）, dopamine（DA）, and norepinephrine（NE） were tested in the brains. Result： The learning and memory ability in group B were lower than that of group A, and the contents of Ach, 5-HT, DA, and NE in the brain tissue also decreased; Compared with group B, the contents of Ach, 5-HT, DA, and NE group C, D and E increased, and the increase extent in group D was more significant than that in group C and E. Conclusion： Ultra-filtration extract from Angelica Sinensis Radix and Hedysari Radix Polybotrys can improve the learning and memory ability of old age rats, which may be related to the increase of the Ach, 5-HT, DA, and NE in the brain tissue, and the effect of 1×105 Da molecular weight group is better than others molecular weight group.