文献类型：期刊文献

英文题名：Recovery and recycling of solvent of counter-current chromatography: The sample of isolation of zeaxanthin in the Lycium barbarum L. fruits

作者：Duan, Wen-Da[1,2,3,4];Quan, Kai-Jun[1,2];Huang, Xin-Yi[1,2,3];Gong, Yuan[1,2,3];Xiao, Sun[1,2,3];Liu, Jian-Fei[1,2,3];Pei, Dong[1,2];Di, Duo-Long[1,2,3]

第一作者：Duan, Wen-Da;段文达

通信作者：Huang, XY[1];Di, DL[1];Huang, XY[2];Di, DL[2]

机构：[1]Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, 18 Tianshui Middle Rd, Lanzhou, Gansu, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, 18 Tianshui Middle Rd, Lanzhou, Gansu, Peoples R China;[3]Univ Chinese Acad Sci, Beijing, Peoples R China;[4]Gansu Univ Tradit Chinese Med, Dept Pharm, Lanzhou, Peoples R China

第一机构：Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, 18 Tianshui Middle Rd, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, 18 Tianshui Middle Rd, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, 18 Tianshui Middle Rd, Lanzhou, Gansu, Peoples R China.

年份：2021

卷号：44

期号：3

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000607589200001)】；

基金：The authors thank the financial support of the Drug Innovation Major Project (2018ZX09711001-001-002), the Key Research and Development Program of Ninxia Hui Autonomous Region (2019BEF02006), Qingdao Municipal Science and Technology Huimin Demonstration Project (Key Technologies and Applications for Large-Scale Separation and Preparation of Zeaxanthin) and the CAS 'Light of West China' Program. In addition, Dr. Xin-Yi Huang also thanks the support from the Youth Innovation Promotion Association CAS (2016369).

语种：英文

外文关键词：counter-current chromatography; Lycium barbarum L; zeaxanthin

摘要：An efficient method of recovering and recycling solvent for counter-current chromatography was established by which zeaxanthin was separated from Lycium barbarum L. fruits. A column with activated carbon combined with high performance counter-current chromatography formed the recovering and recycling solvent system. Using the solvent system of n-hexane-ethyl acetate-ethanol-water (8:2:7:3, v/v) from the references, five injections were performed with an almost unchanged purity of zeaxanthin (80.9, 81.2, 81.5, 81.3, and 80.2% respectively) in counter-current chromatography separation. Meanwhile, the mobile phase reduced by half than conventional counter-current chromatography. By this present method, an effective improvement of counter-current chromatography solvent utilization was achieved.