文献类型：期刊文献

英文题名：Genetic transformation of Gentiana dahurica Fisch by Agrobacterium tumefaciens using zygotic embryo-derived callus

作者：Sun, Shao-Bo[1];Meng, Lai-Sheng[2]

第一作者：孙少伯

通信作者：Sun, SB[1]

机构：[1]GanSu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China;[2]Tongji Univ, Sch Life Sci, Shanghai 200092, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), GanSu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2010

卷号：32

期号：4

起止页码：629

外文期刊名：ACTA PHYSIOLOGIAE PLANTARUM

收录：;Scopus(收录号：2-s2.0-77953869369);WOS:【SCI-EXPANDED(收录号:WOS:000278809800003)】；

语种：英文

外文关键词：Gentiana dahurica Fisch; Somatic embryogenesis; Organogenesis; Glucuronidase (GUS); npt II gene; Agrobacterium tumefaciens

摘要：Gentiana dahurica Fisch is one of four important commercial Radix Gentianae stipulated by the Chinese Pharmacopoeia. We have established a rapid and effective regeneration system using zygotic embryo-derived callus of G. dahurica Fisch. Using this regeneration system, Agrobacterium tumefaciens-mediated transformation of G. dahurica Fisch has been developed. Zygotic embryo-derived callus was infected with an A. tumefaciens strain (GV3130) harboring a pBI121 vector that contained an nptII selective marker gene. In a total of 60 zygotic embryo-derived calli assayed, frequency of calli with nptII gene PCR-positive transgenic plantlets is 5%. Transient glucuronidase (GUS) expression was observed from these transgenic plantlets. Frequency of GUS-positive transgenic plantlets (4/78) was approximately consistent with that of PCR assay (3/60). Our data indicate that we have successfully established a stable G. dahurica Fisch transformation system by A. tumefaciens. In general, this transformation system we have established might be useful for modifying physiological, medicinal and horticultural traits.