文献类型：期刊文献

英文题名：Dioscorea oppositifolia L. Attenuates Weaning-Induced Intestinal Injury by Regulating Oxidative Stress and Apoptosis in Piglets

作者：Shi, Xiongwei[1,2];Ge, Shaoguang[1,2];Wang, Haimin[1,2];Chen, Xiaowang[1,2];Pan, Xiangyi[2];Liu, Chen[2];Qiu, Zhengying[2];Zou, Wenshu[3];Cao, Hao[2];Liu, Yujia[2];Bai, Qiyu[2];Xin, Ruihua[2]

第一作者：Shi, Xiongwei

通信作者：Xin, RH[1]

机构：[1]Gansu Univ Chinese Med, Sch Pharm, Lanzhou 730101, Peoples R China;[2]Chinese Acad Agr Sci, Engn & Technol Res Ctr Tradit Chinese Vet Med Gans, Key Lab Vet Pharmaceut Dev, Minist Agr & Rural Affairs PR Lanzhou,Lanzhou Inst, Lanzhou 730050, Peoples R China;[3]China Acad Chinese Med Sci, Inst Tradit Chinese Med Hlth Ind, Nanchang 330115, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Chinese Acad Agr Sci, Engn & Technol Res Ctr Tradit Chinese Vet Med Gans, Key Lab Vet Pharmaceut Dev, Minist Agr & Rural Affairs PR Lanzhou,Lanzhou Inst, Lanzhou 730050, Peoples R China.

年份：2026

卷号：13

期号：4

外文期刊名：VETERINARY SCIENCES

收录：;Scopus(收录号：2-s2.0-105037323359);WOS:【SCI-EXPANDED(收录号:WOS:001750682600001)】；

基金：This work was supported by the Agricultural Science and Technology Support Project of Gansu Province (KJZC-2024-32), Gansu Haizhi Program Project (Project No. KPZX-010855), Lanzhou Science and technology project (2025-2-21), Key Research and Development fund of Gansu Province (23YFNA0010).

语种：英文

外文关键词：Chinese yam; weaned piglets; intestinal injury; oxidative stress; apoptosis

摘要：Weaning stress is frequently associated with intestinal oxidative stress, inflammatory activation, and epithelial apoptosis in piglets. This study investigated whether dietary supplementation with Chinese yam (Dioscorea oppositifolia L., YAM) alleviates weaning-induced intestinal injury by modulating the oxidative stress-inflammation-apoptosis axis. 48 weaned piglets were assigned to a control diet or diets supplemented with low (1%)/high (2%) doses of YAM. Intestinal morphology, antioxidant capacity, inflammatory signaling, and apoptosis-related markers were assessed, and jejunal transcriptomic profiling was also performed. Supplementing with YAM improved villus architecture and enhanced intestinal antioxidant properties, manifested as increased total antioxidant capacity and reduced malondialdehyde levels. At the molecular level, YAM activated the Keap-1/Nrf2/HO-1 pathway and upregulated the expression of antioxidant-related genes, including superoxide dismutase 2 (SOD2), catalase (CAT), and NAD(P)H quinone dehydrogenase 1 (NQO1), and suppressed NF-kappa B signaling by reducing Myd88 and p-p65 protein levels. In addition, YAM modulated mitochondrial apoptosis by upregulating Bcl-2 and reducing the expression of Bax and Cleaved caspase-3. Transcriptomic analysis identified 1227 differentially expressed genes between the control and high-dose groups (784 upregulated and 443 downregulated). Mechanism-oriented module analysis further confirmed coordinated enhancement of antioxidant pathways alongside suppression of inflammatory and apoptotic gene signatures. These findings demonstrate that dietary YAM supplementation attenuates weaning-associated intestinal injury by rebalancing oxidative stress, inflammatory signaling, and apoptosis-related pathways, thereby supporting its potential application as a functional feed additive in swine production.