文献类型：期刊文献

中文题名：DNA测序建立甘肃当归、大黄种子rRNA基因图谱的研究

英文题名：Studies on Establishing rRNA Gene Map of Angelica sinensis and Rheum palmatum from Gansu by DNA Sequencing

作者：张西玲[1];姬可平[1];李应东[1];陈彻[1];李啸红[1];刘丽莎[1]

第一作者：张西玲

机构：[1]甘肃中医学院,兰州730000

第一机构：甘肃中医药大学

年份：2003

卷号：26

期号：7

起止页码：481

中文期刊名：中药材

外文期刊名：Journal of Chinese Medicinal Materials

收录：MEDLINE(收录号：14650057);CSTPCD;;Scopus(收录号：2-s2.0-1542546123);北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

基金：甘肃省自然科学基金项目ZS0 11 A2 5 0 5 8 Y

语种：中文

中文关键词：当归;大黄;种子;rRNA基因图谱;DNA测序;套式PCR;药用植物

外文关键词：Nested PCR;DNA sequencing;rRNA gene; Angelica sinensis ; Rheum palmatum

摘要：目的 :对甘肃地道性中药材当归、大黄种子中rRNA基因内转录间隔区进行碱基序列测定 ,以期建立从分子水平对中草药种子进行鉴定的一种新方法。方法 :提取当归、大黄种子中核DNA ,利用特异性引物对其rRNA基因内转录间隔区进行套式PCR扩增 ,并将扩增产物进行碱基序列测定。结果 :经琼脂糖凝胶电泳证实 :以上述方法可获得当归、大黄种子DNA中rRNA基因内转录间隔区PCR扩增产物 ;并经测序后得到了当归、大黄种子rRNA基因内转录间隔区的碱基序列 ,且具有明显的差异和可对比性。结论 :不同植物性中药材种子rRNA基因内转录间隔区碱基序列可做为从分子水平进行鉴定的标记。
Objective:To amplify and sequence the internal transcripted spacer of rRNA gene of the seeds of Angelica sinensis and Rheum palmatum for establishing a new method to identify the Chinese herb seeds in molecular level.Methods:rDNA in the seeds was extracted.Specific synthesized primers were applied to amplify the internal transcripted spacer of rRNA gene by nested PCR.And the PCR products were sequenced. Results:PCR results were confirmed by agarose electrophoresis,the above-mentioned methods could achieve the PCR products of interval region of rRNA gene in the seeds,and the sequences of the internal transcripted spacer of rRNA gene in the seeds were acquired by sequencing.There existed marked difference.Conclusion:The base sequences of the internal transcripted spacer of rRNA gene in different plant Chinese seeds can be applied to be identifying marker in molecular level.