文献类型：期刊文献

英文题名：Cryopreservation by PVS2-vitrification causes physiological and histology injuries to dormant buds of Codonopsis pilosula (Franch.) Nannf. while cryo-derived plantlets maintain the genetic stability

作者：Tang, Shunli[1];Zhang, Yanhong[1];Gao, Sufang[1];He, Chunyu[1];Guo, Qingyi[1];Wang, Jinxiu[1];Shen, Sanbao[2]

第一作者：Tang, Shunli

通信作者：Zhang, YH[1]

机构：[1]Gansu Univ Chinese Med, Collaborat Innovat Ctr Nat Med Germplasm Resources, 35 Dingxidonglu Rd, Lanzhou 730000, Peoples R China;[2]Qingshui Cty Agr Technol Extens Stn, Qingshui 741400, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Collaborat Innovat Ctr Nat Med Germplasm Resources, 35 Dingxidonglu Rd, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2026

卷号：164

期号：3

外文期刊名：PLANT CELL TISSUE AND ORGAN CULTURE

收录：;EI(收录号：20261020215738);Scopus(收录号：2-s2.0-105031791352);WOS:【SCI-EXPANDED(收录号:WOS:001706100300002)】；

基金：This work was supported by the National Natural Science Foundation of China (81960683); and the Gansu Provincial University Industry Support Project (2020 C-09).

语种：英文

外文关键词：Antioxidants; Cryoinjury; Cryopreservation; Dormant bud; Genetic stability; Histology

摘要：Vitrification-based cryopreservation holds substantial potential for the large-scale conservation of plant genetic resources. This study investigated the physiological and histological changes in dormant buds of Codonopsis pilosula (Franch.) Nannf. during cryopreservation. At the loading stage, the activity of superoxide dismutase (SOD) and the content of proline were significantly increased, while the activities of catalase (CAT) and peroxidase (POD), as well as the contents of ascorbic acid (AsA) and reduced glutathione (GSH), were markedly decreased. Treatment with Plant Vitrification Solution 2 (PVS2) and freeze-thawing resulted in SOD activity, but had negligible effects on the other aforementioned physiological indicators. Plasmolysis was induced by the loading treatment and was further aggravated after PVS2 exposure. Surviving cells were predominantly distributed in the apical meristem, leaf primordia and the upper segment of the bud axis. Liquid nitrogen (LN) cooling and subsequent freeze-thawing caused extensive cellular damage in the outer and lower segments of the bud axis. In addition, inter-simple sequence repeat (ISSR) markers were employed for the preliminary verification of genetic stability, and no morphological or genomic deoxyribonucleic acid (DNA) variations were detected among the regenerated clones. Collectively, these findings deepen the understanding of the physiological and histological mechanisms underlying the cryopreservation of Codonopsis pilosula dormant buds, and provide a theoretical and technical basis for optimizing cryopreservation protocols for other perennial herbaceous plants.