文献类型：期刊文献

中文题名：归芪白术方对顺铂所致肝毒性小鼠的保护作用

英文题名：Protective effect of GuiQi Baizhu Recipe on hepatotoxicity induced by cisplatin in mice

作者：张丽昕[1];王磊[1];张苡铭[1];张利英[1];刘永琦[1];连小龙[2]

第一作者：张丽昕

机构：[1]甘肃中医药大学基础医学院,兰州730000;[2]甘肃省中药现代制药工程研究院,兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2019

卷号：35

期号：8

起止页码：788

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：甘肃省高校重大疾病分子医学与中医药防治重点实验室开放基金资助项目(ZYX15-16-3);甘肃省科技支撑计划基金资助项目(2012-120FKCA169);陇原青午创新人才扶持计划基金资助项目(20L4-93-72)

语种：中文

中文关键词：归芪白术方;顺铂;肝毒性;减毒作用

外文关键词：Gui Qi Baizhu recipe;cisplatin;hepatotoxicity;detoxification

摘要：目的研究归芪白术方(GBR)对顺铂所致肝毒性的保护作用。方法按照体重将小鼠随机分为5组:空白组,模型组和低、中、高3个剂量实验组,每组10只。以腹腔注射2. 0 mg·mL^(-1)顺铂注射液0. 2 mL制备小鼠肝毒性模型。造模的同时,低、中、高3个剂量实验组灌胃GBR 4. 1,8. 2,16. 4 g·kg^(-1);空白组和模型组灌胃等体积蒸馏水,1次/1 d,连续28 d。用全自动血球分析仪检测小鼠血常规,以全自动生化分析仪检测肝组织中谷草转氨酶(AST)和谷丙转氨酶(ALT)活性。结果空白组、模型组和低、中、高3个剂量实验组的WBC分别为(12. 48±1. 67),(4. 37±0. 30),(6. 36±0. 48),(7. 63±0. 08)和(7. 46±1. 46)109/L-1;这5组的PLT分别为(993. 67±173. 34),(629. 67±134. 11),(839. 67±35. 81),(889. 50±81. 32)和(918. 00±49. 37) g·L^(-1),模型组与空白组比较,WBC和PLT均明显降低,差异均有统计学意义(均P <0. 01);低、中、高3个剂量实验组与模型组比较,WBC和PLT均明显增加,差异均有统计学意义(P <0. 05或P <0. 01)。这5组的ALT分别为(44. 33±14. 36),(77. 33±16. 77),(61. 00±8. 19),(55. 33±7. 51)和(53. 33±10. 26) U·L^(-1);这5组的AST分别为(145. 33±19. 35),(205. 67±33. 26),(196. 67±35. 12),(163. 00±11. 27)和(156. 33±21. 55) U·L^(-1),模型组与空白组比较,ALT和AST均明显增加,差异均有统计学意义(均P <0. 01);高剂量实验组与模型组比较,ALT和AST均明显降低,差异均有统计学意义(均P <0. 05)。结论 GBR能够减弱顺铂对小鼠的肝毒性作用。
Objective To study the protective effect of Guiqi Baizhu Recipe(GBR)on cisplatin-induced hepatotoxicity in mice.Methods Mice were randomly divided into five groups:normal group,model group,experimental-L,M and H groups,with 10 mice in each group.The mice hepatotoxicity model were preparated by injected intraperitoneally with2.0 mg·mL-1 of cisplatin injection 0.2 mL.At the same time of model,the mice in three doses experimental groups were intragastric administration GBR 4.1,8.2,16.4 g·kg-1;the mice in normal and model groups were intragastric administration the same volume of distilled water,1 times a day for 28 days.The blood routine in mice were detected by automatic hematology analyzer.The alanine transaminase(ALT),aspartate transaminase(AST)activity in blood of the liver tissue were detected by fully automatic chemistry analyzer.Results The WBC in normal group,model group,experimental-L,-M and-H groups respectively were(12.48±1.67),(4.37±0.30),(6.36±0.48),(7.63±0.08),(7.46±1.46)109/L-1;the PLT in the five groups respectively were(993.67±173.34),(629.67±134.11),(839.67±35.81),(889.50±81.32),(918.00±49.37)g·L-1;comparison between the model group with normal group,with WBC and PLT decreased,the difference was significant(all P<0.01);comparison experimental-L,-M and-H groups with model group,with WBC and PLT increased,the difference was significant(P<0.05,P<0.01).The ALT in the five groups respectively were(44.33±14.36),(77.33±16.77),(61.00±8.19),(55.33±7.51),(53.33±10.26)U·L-1;the AST in the five groups respectively were(145.33±19.35),(205.67±33.26),(196.67±35.12),(163.00±11.27),(156.33±21.55)U·L-1;comparison between the model group with normal group,the difference was significant with ALT and AST increase(all P<0.01);comparison experimental-H group with model group,the difference was significant with ALT and AST decrease(all P<0.05).Conclusion GBR can reduce the liver toxicity induced by cisplatin in mice.