文献类型：期刊文献

中文题名：党参花药愈伤组织诱导及植株再生体系的建立

英文题名：Establishment of Callus Induction and Plant Regeneration System for Codonopsis pilosula Anthers

作者：孙欢[1];何春雨[1];张延红[1];郭清毅[1];何玉明[1]

第一作者：孙欢

机构：[1]甘肃中医药大学药学院,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2023

卷号：46

期号：2

起止页码：279

中文期刊名：中药材

外文期刊名：Journal of Chinese Medicinal Materials

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；PubMed;

基金：国家自然科学基金项目(81960683);甘肃省高等学校产业支撑计划项目(2020C-09);甘肃省民生科技专项(20CX9NA070);中央引导地方科技发展专项资金项目(30440323);甘肃省知识产权计划项目(21ZSCQ045)

语种：中文

中文关键词：党参;花药培养;花粉发育时期;愈伤组织诱导;植株再生

外文关键词：Codonopsis pilosula(Franch.)Nannf.;Anther culture;Pollen development period;Callus induction;Plant regeneration

摘要：目的:建立党参花药培养的植株再生体系,为党参单倍体育种提供基础研究。方法:以党参花药为外植体,研究不同浓度2,4-D、低温预处理对愈伤组织诱导的影响,并对愈伤组织进行继代培养及体细胞胚胎发生再生植株研究。结果:确定了花粉发育时期与花蕾外部形态的相关性,药瓣比(花药长/花瓣长)为0.83~0.89的党参花蕾,其花粉处于单核中央期或单核靠边期;党参花药愈伤组织诱导的适宜培养基为MS+1.0~2.0 mg/L 2,4-D+蔗糖30 g/L+琼脂8 g/L,诱导率为37.50%~47.50%;低温预处理对愈伤组织诱导的效果不明显;合适的愈伤组织继代、胚状体分化再生植株的培养基分别为MS+0.5 mg/L 2,4-D+蔗糖30 g/L+琼脂8 g/L、MS+0.5 mg/L 2,4-D+椰乳100 g/L+蔗糖30 g/L+琼脂8 g/L。结论:初步建立了通过体细胞胚胎发生再生植株的党参花药培养技术体系,为党参新品种选育奠定了基础。
Objective:To establish a plant regeneration system for the cultivation of Codonopsis pilosula anthers,so as to provide basis for Codonopsis pilosula haploid breeding.Methods:The effects of different concentrations of 2,4-D and cold temperature pretreatment on callus induction were studied using Codonopsis pilosula anthers as explants,and the subculture of callus and somatic embryogenesis and plant regeneration were studied.Results:The relationship between pollen development time and flower bud morphology was determined.The pollen of Codonopsis pilosula buds with the ratio of anther length to petal length of 0.83~0.89 was in the mononuclear central stage or mononuclear sidelined stage.The suitable medium for callus induction was MS+1.0 to 2.0 mg/L,2,4-D withsucrose 30 g/L,agar 8 g/L,the induction rate was 37.50%to 47.50%.The effect of low temperature pretreatment on callus induction was not obvious.The suitable medium for callus subgeneration and embryo differentiation was MS+0.5 mg/L,2,4-D with sucrose 30 g/L,agar 8 g/L;MS+0.5 mg/L,2,4-D with coconut milk 100 g/L,sucrose 30 g/L,agar 8 g/L,respectively.Conclusion:The anther culture system of Codonopsis pilosula regenerated plants by somatic embryogenesis is established,which lays a foundation for the breeding of new varieties of Codonopsis pilosula.