文献类型：期刊文献

中文题名：miR-210在不同病理分级的脑胶质瘤中的表达差异及对肿瘤细胞的作用

英文题名：Analysis of the mechanism of miR-210 in the pathogenesis of nervous system tumors

作者：杜丽[1];付勇[2];杨江河[3];王强[4]

第一作者：杜丽

机构：[1]武警兵团总队医院检验病理科,新疆乌鲁木齐830063;[2]新疆军区总医院病理科,新疆乌鲁木齐830000;[3]新疆军区总医院神经外科,新疆乌鲁木齐830000;[4]甘肃中医学院解剖教研室,甘肃兰州730000

第一机构：武警兵团总队医院检验病理科,新疆乌鲁木齐830063

年份：2020

卷号：28

期号：8

起止页码：1276

中文期刊名：现代肿瘤医学

外文期刊名：Journal of Modern Oncology

收录：CSTPCD

基金：甘肃省自然科学基金项目(编号:16PRJ2A063)。

语种：中文

中文关键词：脑胶质瘤;miR-210;侵袭能力

外文关键词：brain glioma;miR-210;invasive ability

摘要：目的:探讨microRNA-210(miR-210)在脑胶质瘤中的表达,分析其对脑胶质瘤细胞凋亡和侵袭能力的影响。方法:选取2015年3月至2019年4月在我院治疗的56例原发性胶质瘤患者为肿瘤组,同时选取因颅脑损伤行颅内减压和癫痫手术但脑组织正常的23例患者为对照组,取患者少量脑组织,采用实时定量PCR检测miR-210表达。收集肿瘤组患者病理资料,分析不同病理特征患者的miR-210表达差异。对提取的部分胶质瘤细胞,根据是否做miR-210抑制剂转染分为转染和未转染miR-210抑制剂两组,采用流式细胞仪和Transwell法检测两组的胶质瘤细胞凋亡及侵袭能力的变化。结果:肿瘤组的miR-210相对表达量明显高于对照组(P<0.01)。WHO不同分级患者的胶质瘤组织中miR-210的表达比较有统计学意义(P<0.01),其中Ⅳ级患者最高、Ⅰ级患者最低。转染和未转染miR-210抑制剂组的胶质瘤细胞凋亡率比较差异无统计学意义(P>0.05);转染miR-210抑制剂组穿膜细胞数明显低于未转染miR-210抑制剂组(P<0.01)。结论:脑胶质瘤中miR-210呈高表达,其表达量与病理分级升高呈正相关,可促进脑胶质瘤细胞侵袭能力。
Objective:To investigate the expression of microRNA-210(miR-210)in glioma and analyze its effect on apoptosis and invasion of glioma cells.Methods:Fifty-six patients with primary glioma treated in our hospital from March 2015 to April 2019 were selected as the tumor group.At the same time,23 patients with normal brain tissue who underwent intracranial decompression and epilepsy due to craniocerebral injury were selected as the control group.Brain tissue was taken from patients,and the expression of microRNA-210 was detected by real-time quantitative PCR.The pathological data of the patients in the tumor group were collected and the expression of microRNA-210 in patients with different pathological characteristics was analyzed.Some glioma cells were divided into two groups according to whether or not they were transfected with the inhibitor of microRNA-210:Transfection group and non-transfection group.Flow cytometry and Transwell method were used to detect the apoptotic and invasive ability of glioma cells in the two groups.Results:The relative expression of microRNA-210 in the tumor group was significantly higher than that in the control group(P<0.01).The expression of microRNA-210 in glioma tissues of patients with different WHO grades had statistical significance(P<0.01),among which the highest level was in grade IV patients and the lowest level was in grade I patients.There was no significant difference in apoptotic rate of glioma cells between transfected and non-transfected group(P>0.05).The number of migrating cells in the transfected group was significantly lower than that in the non-transfected group(P<0.01).Conclusion:The expression of microRNA-210 is high in glioma,and its expression is positively correlated with the increase of pathological grade,which can promote the invasive ability of glioma cells.