文献类型：期刊文献

中文题名：大黄药材指纹图谱研究

英文题名：Study of Chromatography Fingerprint(CFP) of Dahuang

作者：刘欣[1];胡芳弟[1];封士兰[1];赵健雄[1];李应东[2];徐静汶[1];宋平顺[3]

第一作者：刘欣

机构：[1]兰州医学院;[2]甘肃中医学院;[3]甘肃省药品检验所

第一机构：兰州医学院

年份：2004

卷号：10

期号：3

起止页码：140

中文期刊名：分析测试技术与仪器

外文期刊名：Analysis and Testing Technology and Instruments

基金：甘肃省2002年省长基金项目GSO24 A43 018.

语种：中文

中文关键词：大黄;指纹图谱;高效液相

外文关键词：Dahuang; chromatography fingerprint(CFP); HPLC

摘要：采用高效液相色谱法,HypersilODS柱,甲醇 1.0%冰醋酸为梯度流动相,研究了大黄的指纹图谱,其中大黄酸、大黄素、大黄酚、大黄素甲醚、芦荟大黄素作为为参照物,并对它们进行了含量测定.检测波长:430nm.共找出了23个共有峰,其中的5个峰是参照物,12个样品之间的相似度在90%以上.样品处理方法简单,研究所得的大黄指纹图谱稳定性、重复性好,可作为大黄药材的特征指纹图谱.
Subject and methed: study of Chromatography Fingerprint(CFP) of Dahuang. High-performance liquid chromatography (HPLC) on a Hypersil ODS column with Methanol-1.0%HAC gradient elution. CFP was referenced to emodin,rhein,physcion,aloe-emodin and chrysophanol ,then contents of those were determinted, determination wavelength 430nm. Result:23 common peaks were pointed out and five peaks of those peaks were referenced, their similarity of 12 samples were more than 90%. Conclusion: The handling of samples were simple, the method on chromatography Fingerprint of Dahuang were reliable, good repetition and their similarity accorded with demand on 'the technology guide to experiment studies on chromatography Fingerprint of injection of traditional Chinese medicine(TCM)', issued by the nation pharmacopoeial commission.