文献类型：期刊文献

中文题名：基于SIRT6/NF-κB信号通路研究杠板归总黄酮对急性肝损伤大鼠的保护作用

英文题名：The protective effect of Polygonum perfoliatum L.on acute liver injury in rats was studied based on the SIRT6/NF-κB signaling pathway

作者：连苑宇[1];徐杰[1];朱田田[2];马晓辉[2];曹后康[2];张可锋[1];韦日明[1];高雅[1]

第一作者：连苑宇

机构：[1]桂林医学院,桂林5410041;[2]甘肃中医药大学,兰州730000

第一机构：桂林医学院,桂林5410041

年份：2020

卷号：32

期号：11

起止页码：1818

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：广西自然科学基金面上项目(2017GXNSFAA198326)。

语种：中文

中文关键词：杠板归总黄酮;CCl4;急性肝损伤;SIRT6;NF-κB;炎症反应

外文关键词：total flavonoids from Polygonum perfoliatum L.;CCl4;acute liver injury;SIRT6;NF-κB;inflammatory reaction

摘要：研究杠板归总黄酮(TFP)对四氯化碳(CCl4)致急性肝损伤大鼠的保护作用,并初步探索其作用机制。将70只雌性SD大鼠按体重随机分为7组:正常组、TFP对照组(200 mg/kg)、CCl4模型组、CCl4+水飞蓟素组(120 mg/kg)及CCl4+TFP高、中、低剂量组(200、100、50 mg/kg),每组10只。正常组和CCl4模型组灌胃蒸馏水(8 mL/kg),其余组分别灌胃相应剂量的药物,每天1次,连续10天。末次给药后,除正常组和TFP对照组外,其余各组均腹腔注射12%的CCl4橄榄油溶液(5 mL/kg),建立急性肝损伤模型。禁食不禁水16 h,眼球取血后采集肝组织。生化法检测血清中的肝功能、氧化应激指标;酶联免疫吸附法(ELISA)检测肝组织中炎症因子水平;蛋白质印迹法(Western blot)检测肝组织中SIRT6/NF-κB通路相关蛋白表达水平;HE染色检查肝组织病变程度,免疫组化观察肝组织中p-NF-κB p65的表达水平。结果显示,TFP可改善肝功能指标和肝损伤状况,提高机体抗氧化能力和SIRT6蛋白的表达水平,降低组织中炎症因子和NF-κB等相关蛋白表达。本研究结果表明,TFP对CCl4致急性肝损伤大鼠具有保护作用,其机制可能与SIRT6/NF-κB通路介导的炎症反应和抗氧化有关。
To explore the protective effect of total flavonoid from Polygonum perfoliatum L.(TFP)on acute liver injury induced by carbon tetrachloride(CCl4)in rats and explore its mechanism.The 70 female SD rats were randomly divided into 7 groups according to their body weight:normal group,TFP control group(200 mg/kg),CCl4 model group,CCl4+silymarin group(120 mg/kg)and CCl4+TFP high,middle and low dose groups(200,100,50 mg/kg),with 10 rats in each group.The normal group and the CCl4 model group were treated with distilled water(8 mL/kg),and the other groups were treated with corresponding doses of drugs once a day for 10 days.After the last administration,except the normal group and TFP control group,the other groups were intraperitoneally injected with 12%CCl4 olive oil solution(5 mL/kg)to establish the model of acute liver injury.After fasting for 16 hours,the blood of the eyeball was taken and the liver tissue was collected.The liver function and oxidative stress in serum were detected by biochemical method,the level of inflammatory factors in liver tissue was detected by enzyme-linked immunosorbent assay(ELISA),the expression of SIRT6/NF-κB pathway related protein in liver tissue was detected by Western blot,the pathological changes of liver tissue were detected by HE staining,and the expression level of p-NF-κB p65 in liver tissue was observed by immunohistochemistry.The results showed that TFP could improve the index of liver function and the condition of liver injury,increase the ability of antioxidation and the expression level of SIRT6 protein,and decrease the expression of inflammatory factors and NF-κB and other related proteins in tissue.The results of this study suggest that TFP has a protective effect on CCl4-induced acute liver injury in rats,and its mechanism may be related to the inflammatory response and antioxidation mediated by SIRT6/NF-κB pathway.