文献类型：期刊文献

中文题名：敦煌古医方大补肾汤标准煎液HPLC指纹图谱及多指标成分测定研究

英文题名：Study on HPLC fingerprint and multi-component determination of Dabushen standard decoction

作者：王继龙[1,2];冯晓莉[1,2];魏舒畅[1,2];陈方圆[3];蔺兴遥[2];刘晓霞[1]

第一作者：王继龙

机构：[1]甘肃中医药大学甘肃省中药制药工艺工程研究中心,甘肃兰州730000;[2]敦煌医学与转化教育部重点实验室,甘肃兰州730000;[3]甘肃卫生职业学院,甘肃兰州730207

第一机构：甘肃中医药大学

年份：2021

卷号：52

期号：23

起止页码：7176

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：甘肃省基础研究创新群体项目(1506RJIA034);甘肃省科技计划资助项目(21JR1RA273);甘肃省高等学校创新能力提升项目(2019A-079);甘肃省高等学校创新基金项目(2021A-320);敦煌医学与转化教育部重点实验室开放课题(DHYX19-15)。

语种：中文

中文关键词：大补肾汤;标准煎液;HPLC;指纹图谱;量值传递;敦煌古医方;5-羟甲基糠醛;甘草苷;肉桂酸;甘草酸;五味子醇甲;出膏率;转移率

外文关键词：Dabushen Decoction;standard decoction;HPLC;fingerprint;quality value transmitting;Dunhuang ancient medical prescription;5-hydroxymethylfurfural;liquiritin;cinnamic acid;glycyrrhizic acid;schisandrin;creaming rate;transfer rate

摘要：目的建立敦煌古医方大补肾汤(Dabushen Decoction,DD)标准煎液的HPLC指纹图谱及指标成分含量测定方法,并探寻其量值传递规律。方法制备15批DD标准煎液,建立其HPLC指纹图谱,明确相似度及峰归属,测定指标成分5-羟甲基糠醛、甘草苷、肉桂酸、甘草酸、五味子醇甲的含量、全方出膏率及其转移率,分析饮片-标准煎液间的量值传递关系。结果15批DD标准煎液指纹图谱的相似度均大于0.9,标定了28个共有峰,并指认5个色谱峰(5-羟甲基糠醛、甘草苷、肉桂酸、甘草酸、五味子醇甲)。指标成分5-羟甲基糠醛为1.173~1.404 mg/g,转移率为36.09%~40.79%;甘草苷为2.159~3.413 mg/g,转移率为31.91%~37.41%;肉桂酸为0.317~0.614 mg/g,转移率为32.74%~40.64%;甘草酸为4.175~6.559 mg/g,转移率为20.95%~28.48%;五味子醇甲为0.225~0.275 mg/g,转移率为3.52%~4.29%;出膏率为22.24%~26.75%,转移率为93.14%~111.01%。结论采用指纹图谱、多指标含量及出膏率相结合的评价模式对DD标准煎液进行量值传递分析,科学合理,系统全面,可为后续DD的制剂开发和质量控制提供参考。
Objective To establish the HPLC fingerprint and multi-component determination for standard decoction of Dabushen Decoction(大补肾汤,DD),explore the law of its quality value transfer.Methods By preparing 15 batches of standard decoction of DD based on ancient medical records,the HPLC fingerprints were established,the similarity and attribution of common peaks were defined.The index components content and transfer rate of liquiritin,cinnamic acid,glycyrrhizic acid and schisandrin were measured,the ointment rate of the whole prescription were determined,and the transfer analysis of decoction pieces to substance benchmark was performed.Results In the 15 batches of standard decoction of DD,the similarity of fingerprints was greater than 0.9,28 common peaks were identified and five chromatographic peaks were identified(5-hydroxymethylfurfural,liquiritin,cinnamic acid,glycyrrhizic acid and schisandrin).The content of 5-hydroxymethylfurfural was 1.173—1.404 mg/g,the transfer rate was 36.09%—40.79%.The content of liquiritin was 2.159—3.413 mg/g,the transfer rate was 31.91%—37.41%.The content of cinnamic acid was 0.317—0.614 mg/g,the transfer rate was 32.74%—40.64%.The content of glycyrrhizic acid was 4.175—6.559 mg/g,the transfer rate was 20.95%—28.48%.The content of schisandrin was 0.225—0.275 mg/g,the transfer rate was 3.52%—4.29%.The creaming rate was 22.24%—26.75%,the transfer rate was 93.14%—111.01%.Conclusion The evaluation model of fingerprint combined with multi-index content determination and creaming rate was used to analyze the quantity value transfer of standard decoction,which was scientific,reasonable and systematic,and could provide reference for the subsequent development and quality control of DD.