文献类型：期刊文献

英文题名：Effects of warm needle acupuncture on behavioral pain threshold in a rat model of migraine

作者：Liu, Qiang[1];Qin, Xiaoguang[1];Luo, Chenglin[1];Zhao, Zhongting[1];Xie, Wangjun[1];Du, Xiaozheng[1];Qin, Xin[2]

第一作者：刘强

通信作者：Du, XZ[1];Qin, X[2]

机构：[1]Gansu Univ Tradit Chinese Med, Dept Acupuncture & Moxibust, Lanzhou, Peoples R China;[2]Northwest Minzu Univ, Med Fac, Lanzhou, Peoples R China

第一机构：甘肃中医药大学针灸推拿学院

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Dept Acupuncture & Moxibust, Lanzhou, Peoples R China;[2]corresponding author), Northwest Minzu Univ, Med Fac, Lanzhou, Peoples R China.|[10735db5df93766a96e5b]甘肃中医药大学针灸推拿学院;[10735]甘肃中医药大学;

年份：2025

卷号：16

外文期刊名：FRONTIERS IN NEUROLOGY

收录：;Scopus(收录号：2-s2.0-105019234719);WOS:【SCI-EXPANDED(收录号:WOS:001594114100001)】；

基金：The author(s) declare that financial support was received for the research and/or publication of this article. Thanks Gansu Provincial Department of Education 2020 Innovation Ability Improvement Project: The regulation mechanism of PKC epsilon pathway in migraine model rats under the intervention of Wentong acupuncture based on the theory of pain sensitivity. PKC pathway regulation mechanism (2020B-52) supporting and promoting this research work.

语种：英文

外文关键词：needle warming acupuncture; migraine; ET-1 protein; 5-HT; CGRP

摘要：Background: As a traditional Chinese treatment method, acupuncture has a long history in the treatment of migraine and a rich literature base. As a traditional acupuncture technique, warm needle acupuncture has clearly demonstrated clinical efficacy in the treatment of migraine, although its mechanism of action remains unclear. This study aimed to evaluate the efficacy and safety of warm needle acupuncture in the treatment of migraine in a rat model of migraine by comparing pain threshold and analgesic-related factors among different groups. Methods: Thirty Sprague-Dawley rats were randomly divided into control, model, and acupuncture groups, with 10 animals in each group. On day 8, rat migraine models were established by subcutaneous injection of nitroglycerin. The acupuncture group was bilaterally needled once per day at Fengchi, Baihui, Fengfu, and Hegu. Behavioral changes and biochemical alterations among the rats before and after modeling were observed for 7 consecutive days after treatment. The expression of Endothelin-1 (ET-1), interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-alpha) in the brain stem of the animals were assessed using ELISA. Expression levels of calcitonin gene-related peptide (CGRP) mRNA were determined using real-time quantitative polymerase chain reaction techniques. Results: After treatment, the expression of ET-1, IL-6, TNF-alpha, and CGRP in the brain stem of the model group was significantly increased compared with that of the control group (p < 0.05), while the expression of IL-6, TNF-alpha, and CGRP in the brain stem of the acupuncture group was decreased. Conclusion: Warm needle acupuncture reduced ET-1 protein levels and promoted the expression of neurotransmitters, such as 5-hydroxytryptamine, in migraine rat model animals. In addition, needle warming acupuncture decreased IL-6, TNF-alpha, and CGRP levels in the brain stem of the migraine group, which may explain its mechanism of action in treating migraine.