文献类型：期刊文献

中文题名：基于TLR4/MyD88/NF-κB信号通路探讨芪附益心颗粒对心力衰竭大鼠心脏炎症状态的影响

英文题名：Modulation of cardiac inflammation by Qifu Yixin Granules in rats with heart failure via TLR4/MyD88/NF-κB signaling pathway

作者：李倩蓉[1,2];支晓东[1,2,3];蒋兵[1,2];王春玲[1,2];任春贞[1,2];赵信科[1,2,3];刘凯[4];李应东[1,2,3]

第一作者：李倩蓉

机构：[1]甘肃中医药大学中西医结合学院,甘肃兰州730000;[2]甘肃省中医药防治慢性病重点实验室,甘肃兰州730000;[3]甘肃中医药大学附属医院,甘肃兰州730000;[4]甘肃医学院临床医学院,甘肃平凉744000

第一机构：甘肃中医药大学中西医结合学院

年份：2025

卷号：47

期号：8

起止页码：2535

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金资助项目(82260869,82360926);甘肃教育解绑挂帅项目(2021jyjbgs-03);甘肃省中医药防治重大疾病专项(CZKZD-2018-02);甘肃省科技重大专项(20ZD7FA002)。

语种：中文

中文关键词：芪附益心颗粒;心力衰竭;炎症;TLR4/MyD88/NF-κB信号通路

外文关键词：Qifu Yixin Granules;heart failure;inflammation;TLR4/MyD88/NF-κB signaling pathway

摘要：目的探讨芪附益心颗粒对心力衰竭大鼠心脏炎症状态的影响。方法大鼠以腹腔注射阿霉素法诱导6周建立慢性心力衰竭(CHF)模型,将造模成功的大鼠随机分为模型组、卡托普利组(22.5 mg/kg)和芪附益心颗粒低、中、高剂量组(2.84、5.67、11.34 g/kg),另取正常不造模的大鼠作为空白组。观察大鼠体质量变化;超声心动图检测心功能;ELISA法检测血清NT-proBNP、TNF-α、IL-6、IL-1、CRP水平;HE及Masson染色观察心肌组织形态学变化;免疫组化及Western blot法检测心肌组织TLR4、MyD88、NF-κB蛋白表达;RT-qPCR法心肌组织TLR4、MyD88、NF-κB mRNA表达。结果与空白组比较,模型组大鼠体质量和LVEF、LVFS值降低(P<0.01),LVEDD、LVESD值和血清NT-proBNP、TNF-α、IL-6、IL-1、CRP水平均升高(P<0.01);心肌组织有炎性细胞聚集,胶原纤维沉积增加(P<0.01);心肌组织TLR4、MyD88、NF-κB蛋白和mRNA表达均升高(P<0.01)。与模型组比较,卡托普利组和芪附益心颗粒中、高剂量组大鼠体质量和LVEF、LVFS值升高(P<0.05,P<0.01),LVEDD、LVESD值和血清NT-proBNP、TNF-α、IL-6、IL-1、CRP水平降低(P<0.05,P<0.01);炎性浸润减少,胶原纤维沉积减少(P<0.05,P<0.01);心肌组织TLR4、MyD88、NF-κB蛋白和mRNA表达降低(P<0.05,P<0.01)。结论芪附益心颗粒可减轻阿霉素致慢性心力衰竭大鼠心脏炎症,改善心功能,其作用机制可能与抑制TLR4/MyD88/NF-κB信号通路活化有关。
AIM To investigate the effects of Qifu Yixin Granules on cardiac inflammation in a rat model of heart failure.METHODS The rats were induced into chronic heart failure(CHF)models by 6-week intraperitoneal injection of doxorubicin followed by the random assignment of the successful rat models into the model group,the captopril group(22.5 mg/kg),and the low-dose,medium-dose,and high-dose Qifu Yixin Granules groups(2.84,5.67,11.34 g/kg),in contrast to the normal rats of the blank group.The rats had their body weight monitored;their cardiac function assessed by echocardiography;their serum levels of NT-proBNP,TNF-α,IL-6,IL-1 and CRP measured by ELISA;their cardiac morphological alterations observed by HE and Masson staining;their cardiac protein expressions of TLR4,MyD88 and NF-κB detected by immunohistochemistry and Western blot;and their cardiac mRNA expressions of TLR4,MyD88 and NF-κB measured by RT-qPCR.RESULTS Compared to the blank group,the model group exhibited significantly reduced body weight,LVEF and LVFS(P<0.01),alongside significantly elevated LVEDD,LVESD,and serum concentrations of NT-proBNP,TNF-α,IL-6,IL-1 and CRP(P<0.01).Additionally,the model group displayed greater myocardial inflammatory cell aggregation,increased collagen deposition(P<0.01);and upregulated myocardial protein and mRNA expressions of TLR4,MyD88 and NF-κB(P<0.01).Compared to the model group,the groups intervened with captopril or medium/high dose Qifu Yixin Granules demonstrated significantly increased body weight,LVEF and LVFS(P<0.05,P<0.01);significantly reduced LVEDD,LVESD,and serum levels of the aforementioned indicators(P<0.05,P<0.01);mitigated inflammation and collagen deposition(P<0.05,P<0.01);and downregulated myocardial protein and mRNA expressions of TLR4,MyD88 and NF-κB(P<0.05,P<0.01).CONCLUSION Qifu Yixin Granules attenuate cardiac inflammation and improve cardiac function in doxorubicin-induced CHF rats;this therapeutic effect is mediated by inhibiting the activation of the TLR4/MyD88/NF-κB signaling pathway.