文献类型：期刊文献

中文题名：四逆散对创伤后应激障碍大鼠血清分子表达的作用

英文题名：Function of Sinisan on serummolecular expression in rats with post-traumatic stress disorder

作者：曹瑞[1,2];牛江涛[1,2];边甜甜[1,2];司昕蕾[1,2];李越峰[1,2]

第一作者：曹瑞

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃中医药大学甘肃省中药质量与标准研究重点实验室,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2019

卷号：35

期号：3

起止页码：257

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金资助项目(81460611);教育部科学技术研究重点基金资助项目(212186);甘肃省自然科学基金资助项目(2010:1010RJZA212; 2014:145RJZA076);甘肃省财政厅高校基本科研业务费专项基金资金项目(2013-2);兰州市科技计划基金资金项目(2014-1-188);甘肃省中医药管理局科研课题基金资金项目(GZK-2015-57);甘肃省中药质量与标准研究重点实验室培育基地开放研究基金资助项目(ZYZL17-008)

语种：中文

中文关键词：四逆散;创伤后应激障碍;血清;高效液相色谱法

外文关键词：Sinisan;post-traumatic stress disorder;serum;high performance liquid chromatography

摘要：目的研究四逆散干预创伤后应激障碍大鼠的分子生物学机制。方法按照体重将SD大鼠随机分为5组:空白对照组、模型组、阴性对照组、阳性对照组、实验组,每组10只。除空白对照组外,其他4组动物均复制模型。空白对照组与模型组均不接受药物干预。阴性对照组灌胃等体积0. 9%Na Cl,阳性对照组灌胃盐酸帕罗西汀溶液0. 42 mg·mL^(-1),实验组灌胃四逆散水煎液(含生药0. 24 g·mL^(-1))。于应激造模前1 h灌胃给药10 mL·kg^(-1),每天灌服1次,共计7 d。于末次给药5 h后,采集血清。用高效液相色谱仪测定各组大鼠血清色谱峰。色谱条件:Agilent HC-C18(4. 6 mm×250 mm,5μm);流速:1 mL·min^(-1);流动相A为乙腈,B为0. 05%磷酸水,梯度洗脱;检测波长:220 nm;柱温:30℃。结果与空白对照组6号、7号、8号、9号及10号的峰面积(225. 92±73. 45),(128. 33±41. 56),(208. 65±27. 19),(88. 84±14. 67)和(27. 35±6. 24) m AU×min比较,模型组6号、7号、8号峰面积(120. 62±4. 39),(55. 82±7. 76)和(49. 48±6. 95) m AU×min明显降低;而模型组的9号、10号峰面积(107. 45±20. 04)和(63. 82±7. 19) m AU×min明显升高,差异均有统计学意义(均P <0. 05)。阴性对照组的6号、7号、8号、9号及10号峰面积分别为(154. 22±8. 88),(47. 72±5. 27),(47. 64±6. 73),(109. 91±19. 57)和(70. 66±6. 82) m AU×min,阴性对照组与模型组比较,差异均无统计学意义(均P> 0. 05)。阳性对照组和实验组的6号峰面积分别为(408. 98±32. 95)和(189. 48±122. 14) m AU×min,这2组的7号峰面积分别为(113. 04±11. 50)和(86. 78±53. 92) m AU×min,这2组的8号峰面积分别为(110. 08±10. 44)和(144. 37±16. 74) m AU×min,这2组的9号峰面积分别为(55. 22±8. 95)和(76. 73±9. 54) m AU×min,这2组的10号峰面积分别为(34. 74±9. 56)和(28. 68±7. 82) m AU×min,这2组与阴性对照组比较,差异均有统计学意义(均P <0. 05)。实验组与阳性对照组比较,新出现了5号峰,提示该峰可能是四逆散的内在化学成分或其代谢产物。结论四逆散对大鼠血清分子表达有明显的正向调节作用。
Objective To study the molecular biological mechanism of Sinisan on intervention in rats with post-traumatic stress disorder( PTSD). Methods Sprague Dawley rats were divided equally into 5 groups: blank control group,model group,negative control group,positive control group and experimental group,10 rats in each group. Except for the blank control group,the other 4 groups of animals were replicated the model. The control group and model group did not receive drug intervention. The negative control group was given equal volume 0. 9% NaCl,the positive control group was given paroxetine hydrochloride solution 0. 42 mg·mL-1,and the experimental group was given Sinisan Decoction( containing 0. 24 g·mL-1 crude drug). The rats were given 10 mL·kg-1 at one hour before the stress modeling,and 1 times a day for 7 d. At the end of 5 hours after the last administration,the serum were collected. Fingerprints were used to compare the difference peaks of serumin the rats with PTSD. High performance liquid chromatography conditions were as follows: the column was Agilent HC-C18( 4. 6 mm × 250 mm,5 μm),with a mobile phase composition of acetonitrile( phase A) and 0. 05% phosphorus acid water( phase B);at a flow of 1 mL·min-1,the wavelength of detection was 220 nm and column warm was kept at 30 ℃. Results Compared with the peak areas of 6 th( 225. 92 ± 73. 45) m AU × min,7 th( 128. 33 ± 41. 56) m AU × min,8 th( 208. 65 ± 27. 19) m AU × min,9 th( 88. 84 ± 14. 67) m AU × min,10 th( 27. 35 ± 6. 24) m AU × min in blank control group,the peak areas in model group of 6 th( 120. 62 ± 4. 39) m AU × min,7 th( 55. 82 ± 7. 76) m AU × min,8 th( 49. 48 ± 6. 95) m AU × min were significantly decreased( all P < 0. 05),peak areas of 9 th( 107. 45 ± 20. 04) m AU × min,10 th( 63. 82 ± 7. 19) m AU × min were significantly higher( all P < 0. 05). Compared with model group,the peak areas of 6 th( 154. 22 ± 8. 88) m AU ×min,7 th( 47. 72 ± 5. 27) m AU × min,8 th( 47. 64 ± 6. 73) m AU × min,9 th( 109. 91 ± 19. 57) m AU × min,10 th( 70. 66 ± 6. 82) m AU × min in negative control group with did not change significantly( all P > 0. 05). Compared with negative control group,the peak areas 6 th of positive control group and experimental group were( 408. 98 ± 32. 95),( 189. 48 ± 122. 14) m AU × min,7 th of the 2 groups were( 113. 04 ± 11. 50),( 86. 78 ± 53. 92) m AU × min,8 th of the 2 groups were( 110. 08 ± 10. 44),( 144. 37 ± 16. 74) m AU × min in positive control group and experimental group were significantly higher( all P < 0. 05),while 9 th( 55. 22 ± 8. 95),( 76. 73 ± 9. 54) m AU × min,10 th( 34. 74 ± 9. 56),( 28. 68 ± 7. 82) m AU × min were significantly decreased( all P < 0. 05). Compared with the positive control group,a new peak No. 5 appeared in the experimental group,suggesting that the peak may be the internal chemical constituents or metabolites of Sinisan. Conclusion The Sinisan has significant positive effect on serum molecular expression.