文献类型：期刊文献

中文题名：两种检测器测定党参中苍术内酯Ⅲ含量的比较

英文题名：Comparison of the contents of atractylenoide III in Dangshen that two detectors determined

作者：李中华[1];胡燕[2];刘强[3]

第一作者：李中华

机构：[1]荣成市人民医院;[2]兰州大学;[3]甘肃中医学院

第一机构：荣成市人民医院

年份：2005

卷号：22

期号：5

起止页码：45

中文期刊名：甘肃中医学院学报

外文期刊名：Journal of Gansu College of Traditional Chinese Medicine

语种：中文

中文关键词：蒸发光散射检测器;RP-HPLC;二级管阵列检测器;苍术内酯Ⅲ;含量测定

外文关键词：ELSD ; RP-high performance liquid chromatography ; PAD ; atractylenolide Ⅲ ; determination of content

摘要：目的:以蒸发光散射检测器(ELSD)测定党参中苍术内酯Ⅲ的含量,并与二级管阵列检测器(PAD)测定结果进行比较。方法:采用RP-HPLC法,Hypersil ODS 2柱(5μm,4.6 mm×250 mm),甲醇-水为流动相(67∶33),流速1.0 mL/m in,柱温30℃。结果:用ELSD检测党参药材中苍术内酯Ⅲ的回归方程为A=1.30×104C-2.33×104,相关系数r=0.9938,平均回收率为103.65%,RSD为0.93%。结论:以ELSD测得的党参药材中苍术内脂Ⅲ的含量与PAD测得的党参药材中苍术内脂Ⅲ的含量基本一致,以ELSD RP HPLC法测定党参药材中苍术内脂Ⅲ的含量的方法简便、易行、准确、可靠。
Objective： To determinate the content of atractylenoide Ⅲ in Dangshen by ELSD and PAD, and compare the results. Methods： Adopt RP-high performance liquid chromatography （HPLC） and Hypersil ODS -2 column （5 μm,4.6 mm ×250 mm） . Mobile phase was methanol-water（67 ： 33）, flow-rate was 1.0mlJ min, column temperature was 30 ℃. Results： Linear equation of atractylenolide Ⅲ in Dangshen by ELSD was A = 1.30 × 10 4C -2.33 × 10 4. Relative coefficient was 0. 9938. The average recovery was 103.65 percent and relative standard deviation was 0. 93 percent. Conclusion ： The content of atractylenolide Ⅲ by ELSD and PAD was unanimous basically. The method by ELSD was simple, reliable, accurate and practical.