文献类型：期刊文献

中文题名：甘肃产四倍体黄芩与二倍体黄芩止血作用的比较

英文题名：Comparision of Hemostatic Effects Between Tetraploid and Diploid Scutellariae Radix Cultivated in Gansu Province

作者：杨志军[1];杨秀娟[1];耿广琴[1];杜弢[1,2]

第一作者：杨志军

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃中医药大学药用植物遗传育种研究所,兰州730000

第一机构：甘肃中医药大学

年份：2015

卷号：32

期号：10

起止页码：1184

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：甘肃省中药材产业科技攻关项目(GYC12-05);甘肃省高校基本科研业务费项目(BH2012-020)

语种：中文

中文关键词：黄芩;四倍体;二倍体;止血作用

外文关键词：Scutellariae Radix; tetraploid; diploid; hemostatic effects

摘要：目的比较甘肃产四倍体黄芩与二倍体黄芩的止血作用。方法将70只SD大鼠随机分为正常对照组、模型对照组、阳性对照组、二倍体黄芩生品组、二倍体黄芩炭品组、四倍体黄芩生品组、四倍体黄芩炭品组。除正常对照组外,其余各组给予5%的乙醇自由饮用,并给予干姜水煎液灌胃,连续造模15 d。第15天造模后7 h,各给药组按10 g·kg-1剂量灌胃给予相应黄芩水煎液,连续给药7 d,检测各组大鼠出血时间(bleeding time,BT)、血浆复钙时间(plasma recalcification time,PRT)、凝血功能4项。结果与正常对照组比较,模型对照组大鼠BT、PRT、凝血酶原时间(PT)、凝血酶时间(thrombin time,TT)、活化部分凝血活酶时间(activated partial thromboplastin time,APTT)显著延长(P<0.05),纤维蛋白原(fibrinogen,FIB)含量显著增加(P<0.05);与模型对照组比较,各药物组大鼠BT、PRT、PT、TT、APTT时间均显著缩短(P<0.05),FIB含量显著降低(P<0.05);与同一品种生品组比较,二倍体黄芩炭品、四倍体黄芩炭品组大鼠BT、PRT、PT、TT、APTT时间均显著缩短(P<0.05)。结论甘肃产四倍体黄芩与二倍体黄芩水煎液止血作用无显著差异,而炭品的止血效果显著优于同品种生品。
OBJECTIVE To compare the hemostatic effects of tetraploid and diploid Scutellariae Radix cultivated in Gansu province. METHODS Seventy rats were randomly divided into 7 groups： normal control group, model control group, positive control group, diploid and tetraploid Scutellariae Radix groups, carbonized diploid and tetraploid Scutellariae Radix groups. The blood heating and bleeding model was established by giving decoction of Zingiberis Rhizoma combined with 5% ethanol for 15 d. Then each group adopted corresponding drugs for 7 d. Hemostatic effects of tetraploid and diploid Scutellariae Radix were compared by detecting bleeding time（BT）, plasma recalcification time（PRT） and 4 items of blood coagulation function. RESULTS Compared with normal control group, BT, PRT, prothrombintime（PT）, thrombin time（TT）, activated partial thromboplastin time（APTT） of rats in model control group were prolonged significantly（P〈0.05） and the content of fibrinogen（FIB） increased significantly（P〈0.05）. BT, PRT, PT, TT, APTT and FIB of rats in Scutellariae Radix groups were remarkably lower than those in model control groups（P〈0.05）. Compared with the same varieties of Scutellariae Radix, BT, PRT, PT, TT, APTT of rats in carbonized diploid and tetraploid Scutellariae Radix groups shortened significantly（P〈0.05）. CONCLUSION The hemostatic effects of tetraploid and diploid Scutellariae Radix decoction have no significant difference,but the carbonized diploid and tetraploid Scutellariae Radix have better hemostatic effects than the Scutellariae Radix.