文献类型：期刊文献

中文题名：扶正抑瘤方对胶质瘤微环境下BMSC端粒酶及P53表达的影响

英文题名：Effects of Fuzheng Yiliu Fang(扶正抑瘤方)on Telomerase Activity and P53 Expression in BMSCs in C6 Micro-environment

作者：吴建军[1];曾龙[2];刘永琦[1];岳嘉[1];马占军[2];王转玲[2];周淑宁[2];王学习[2]

第一作者：吴建军

机构：[1]甘肃中医药大学公共卫生学院中医预防医学研究所,甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃兰州730000;[2]兰州大学基础医学院中西医结合研究所,甘肃兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）|甘肃中医药大学公共卫生学院

年份：2017

卷号：24

期号：1

起止页码：19

中文期刊名：中国中医药科技

外文期刊名：Chinese Journal of Traditional Medical Science and Technology

基金：甘肃省中医药管理局项目(GZK-2013-4);甘肃省财政厅科研业务费项目(BH-2013-23)

语种：中文

中文关键词：胶质瘤微环境;骨髓间充质干细胞;端粒酶;P53蛋白;扶正抑瘤方

外文关键词：C6 micro - environment; bone marrow mesenchymal stem cells ; teiomerase; P53 protein; FuzhengYiliu Fang

摘要：目的:观察扶正抑瘤方对胶质瘤(C6)微环境下骨髓间充质干细胞(BMSCs)的影响及作用机制。方法:扶正抑瘤方连续灌胃大鼠并提取大鼠含药血清,利用Transwell小室建立C6与BMSCs共培养环境,BMSCs分成4组进行干预。第1组:BMSCs空白对照组;第2组:BMSCs+扶正抑瘤方含药血清;第3组:BMSCs+C6细胞;第4组:BMSCs+C6细胞+扶正抑瘤方含药血清。通过四甲基偶氮唑蓝(MTT)法检测BMSCs的增殖情况,PCR法检测BMSCs的端粒酶活性,蛋白免疫印迹(Western blot)检测BMSCs中P53蛋白表达。结果:共培养第4组BMSCs的增殖和端粒酶活性比第3组受到较强的抑制(P<0.05);但第4组BMSCs的P53蛋白表达比第3组增高(P<0.05);第1组和第2组BMSCs的增殖、端粒酶活性和P53蛋白的表达均无明显差异(P>0.05)。结论:扶正抑瘤方含药血清对胶质瘤环境下的BMSCs增殖具有抑制作用,其机制可能与抑制BMSCs的端粒酶活性并增加P53表达有关,对BMSCs本身没有影响。
Objective： To observe the effects of Fuzheng Yiliu Fang （FZYLF） on bone marrow mesenchy- mal stem ceils （BMSCs） in C6 micro - environment and explore the mechanism. Methods： Serum containing Fuzheng Yiliu Fang was prepared by ig FZYLF for 3 days （6 times）, （：6 and BMSCs cocuhured micro - environ- ment was built by using Transwell chamber, experiment was divided in 4 groups, 1 ） BMSCs （control） group, 2） BMSCs ＋ serum containing FZYLF, 3 ） BMSCs ＋ C6 cells,4）BMSCs ＋ （：6 ceils ＋ serum containing FZYLF. The proliferation of BMSCs was detected by MTY method, telomerase activity of BMSCs was determined by trap - PCR technique ,P53 protein expression in BMSCs was tested by Western blot. Results： The proliferation of BM- SCs and telomerase activity were inhibited obviously in the fourth group （ BMSCs ＋ C6 ceils ＋ serum containing FZYLF）to compare with the third group（ BMSCs ＋ C6 cells） ,but the expression of P53 protein in BMSCs was enhanced in the fourth group and that was higher than that of the third group （ P 〈 0. 05 ）. All these （ prolifera- tion of BMSCs, telomerase activity and 1〉53 protein expression）were no significant changes in the first （BMSCs） and the second （BMSCs ＋ serum containing FZYLF） group, there were no significant differences between the two groups （P 〉 0. 05 ）. Conclusion： Serum containing FZYLF can inhibit the proliferation of BMSCs in C6 micro - environment, the mechanism is related to inhibiting telomerase activity and enhancing P53 expression.