文献类型：期刊文献

英文题名：Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer

作者：Wang, Wen-Jie[1,2,4];Guo, Chang-An[1,4,5];Li, Rui[1,2];Xu, Zi-Peng[1,3,4];Yu, Jian-Ping[1,3];Ye, Yan[4];Zhao, Jun[2];Wang, Jing[3,4,6];Wang, Wen-An[3,4,6];Zhang, An[3,4,6];Li, Hong-Tao[3];Wang, Chen[1,2];Liu, Hong-Bin[1,3]

第一作者：Wang, Wen-Jie

通信作者：Liu, HB[1];Liu, HB[2]

机构：[1]Lanzhou Univ, Clin Med Coll 2, Lanzhou 730030, Gansu, Peoples R China;[2]Lanzhou Univ, Dept Gen Surg, Hosp 2, Lanzhou 730030, Gansu, Peoples R China;[3]Chinese Peoples Liberat Army, Hosp Joint Logist Support Force 940, Dept Gen Surg, Lanzhou 730050, Gansu, Peoples R China;[4]Key Lab Stem Cells & Gene Drugs Gansu Prov, Lanzhou 730050, Gansu, Peoples R China;[5]Lanzhou Univ, Dept Emergency, Hosp 2, Lanzhou 730030, Gansu, Peoples R China;[6]Gansu Univ Chinese Med, Clin Med Coll, Lanzhou 730000, Gansu, Peoples R China

第一机构：Lanzhou Univ, Clin Med Coll 2, Lanzhou 730030, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Clin Med Coll 2, Lanzhou 730030, Gansu, Peoples R China;[2]corresponding author), Chinese Peoples Liberat Army, Hosp Joint Logist Support Force 940, Dept Gen Surg, Lanzhou 730050, Gansu, Peoples R China.

年份：2019

卷号：11

期号：15

起止页码：5829

外文期刊名：AGING-US

收录：;Scopus(收录号：2-s2.0-85070938626);WOS:【SCI-EXPANDED(收录号:WOS:000481655500039)】；

基金：This study was supported by Cuiying Graduate Supervisor Applicant Training Program of Lanzhou University Second Hospital, Huimin plan of Ministry of Science and Technology of China (2012GS620101), and Major Projects of Science and Technology of Gansu Province (2011GS04390).

语种：英文

外文关键词：gastric cancer; cancer susceptibility 19; progression; prognosis; weighted gene co-expression network analysis

摘要：Evidence indicates that aberrantly expressed long non-coding RNAs (lncRNAs) are involved in the development and progression of advanced gastric cancer (AGC). Using RNA sequencing data and clinical information obtained from The Cancer Gene Atlas, we combined differential lncRNA expression profiling and weighted gene co-expression network analysis to identify key lncRNAs associated with AGC progression and prognosis. Cancer susceptibility 19 (CASC19) was the top hub lncRNA among the lncRNAs included in the gene module most significantly correlated with AGC's pathological variables. CASC19 was upregulated in AGC clinical samples and was significantly associated with higher pathologic TNM stage, pathologic T stage, lymph node metastasis, and poor overall survival. Multivariable Cox analysis confirmed that CASC19 overexpression is an independent prognostic factor for overall survival. Furthermore, quantitative real-time PCR assay confirmed that CASC19 expression in four human gastric cancer cells (AGS, BGC-823, MGC-803, and HGC-27) was significantly upregulated compared with human normal gastric mucosal epithelial cell line (GES-1). Functionally, CASC19 knockdown inhibited GC cell proliferation and migration in vitro. These findings suggest that CASC19 may be a novel prognostic biomarker and a potential therapeutic target for AGC.