文献类型：期刊文献

中文题名：针刺对创伤后应激障碍大鼠行为及学习记忆功能的影响

英文题名：Acupuncture Intervention Improves Behavior Reactions and Learning-memory Ability in Post-traumatic Stress Disorder Rats

作者：赵怡坤[1];韩雅迪[1];张彦峰[1];朱田田[1];马重兵[1];赵中亭[1];严兴科[1]

第一作者：赵怡坤

机构：[1]甘肃中医药大学针灸推拿学院,兰州730000

第一机构：甘肃中医药大学针灸推拿学院

年份：2018

卷号：43

期号：9

起止页码：562

中文期刊名：针刺研究

外文期刊名：Acupuncture Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；PubMed;

基金：国家自然科学基金(No.81460744);甘肃省自然科学研究基金计划项目(No.1308RJZA 150);中国博士后科学基金第62批面上项目(No.2017 M623269)

语种：中文

中文关键词：针刺;创伤后应激障碍;焦虑样行为;自主活动;学习-记忆能力

外文关键词：Acupuncture;Post-traumatic stress disorder;Anxiety-like behavior;Locomotor activity;Learning-memory ability

摘要：目的:观察针刺对创伤后应激障碍(PTSD)大鼠的行为及学习记忆功能的影响。方法:雄性SD大鼠60只随机分为空白对照组、模型组、抓取组、帕罗西汀组、针刺组,每组12只。采用"幽闭+电击"法制备PTSD模型。帕罗西汀组予盐酸帕罗西汀溶液灌胃治疗,针刺组予针刺"百会"及单侧"内关""神门""太冲",各组均每日治疗1次,共12d。观察各组大鼠旷场实验水平跨格数和垂直次数、Morris水迷宫逃避潜伏期和平台穿越次数及新物体识别应用指数(DI)。结果:旷场实验中模型组、抓取组较空白对照组水平跨格数及垂直次数减少(P<0.05),粪便粒数增多(P<0.05)。与模型组、抓取组相比,两治疗组大鼠水平跨格数及垂直次数显著增多(P<0.05),粪便粒数显著减少(P<0.05)。Morris水迷宫实验显示,与空白对照组比较,模型组及抓取组逃避潜伏期延长(P<0.05);与模型组、抓取组相比,两治疗组大鼠逃避潜伏期显著缩短(P<0.05),平台及有效区域穿越次数显著增加(P<0.05)。新物体识别实验显示,与空白对照组相比,模型组与抓取组DI降低(P<0.05);与模型组、抓取组相比,两治疗组DI均有所增加(P<0.05)。结论:针刺能有效改善PTSD模型大鼠异常的行为改变,恢复受损的学习记忆功能。
Objective To observe the therapeutic effect of acupuncture in the treatment of post-traumatic stress disorder （PTSD）-induced abnormal behavior reactions and learning-memory ability in rats with traumatic injury. Methods Sixty male SD rats were randomly divided into control, model, model ＋ animal capturing （capturing）, medication and acupuncture groups （n = 12 rats in each）. The PTSD model was established by ＂electric shock plus incarceration＂ method. Acupuncture was applied to ＂Baihui＂ （GV 20）, and unilateral ＂Neiguan＂ （PC 6）, ＂Shenmen＂ （HT 7） and ＂Taichong＂ （LR 3） once daily for 12 days. The rats in the medication group were treated by gavage of Paroxetine Hydrochloride solution （0.42 mg/mL）, once daily for 12 days. The open field test containing horizontal （crossing grid lines） and rearing tests was performed for examining the rats＇ locomotor activity and anxiety-like behavior; and location navigation （escape latency） and special probe tests （platform quadrant crossing times） of Morris water maze tasks were detected for assessing the rats＇ learning-memory ability. On day 12 of the experiments, the rats were submitted to 3 consecutive sessions of open field tests for observing the time of familiar objects （TF） and the time of novelty object （TN） of exploration in 5 min （an object-location and an object-recognition tasks）, followed by calculating the discrimination index [DI= （TN-TF）/（TN＋TF）×100%]. Results After modeling, compared with the control group, the numbers of crossed grids and rearing, and DI were significantly decreased （P〈0.05）, while the number of fecal pellets and escape latency were sig- nificantly increased in both the model and capturing groups （P〈0.05）. After the intervention, the number of both crossed grids and rearing, platform quadrant crossing times, and DI were considerably increased （P〈0.05）, and the rats＇ escape latency and fecal pellet number were obviously decreased in both medication and acupuncture groups relevant to the model and capturing groups （P〈0.05）. No significant differences were found between the acupuncture and medication groups in the above mentioned 5 indexes （P〉0.05）. Conclusion Acupuncture can effectively reduce anxiety-like behavior and improve the impaired learning- memory ability in PTSD rats.