文献类型：期刊文献

中文题名：脾肾阳虚型溃疡性结肠炎大鼠模型的建立

英文题名：Preparation of a rat model of ulcerative colitis with spleen and kidney Yang deficiency

作者：吴玉泓[1,2];许雅清[1];李海龙[1,2];程小丽[1];明海霞[1];张磊[1];刘峰林[1];王瑞群[1]

第一作者：吴玉泓

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃省中医方药挖掘与创新转化重点实验室,甘肃省中药新产品创制工程实验室,兰州730000

第一机构：甘肃中医药大学

年份：2016

卷号：24

期号：2

起止页码：116

中文期刊名：中国实验动物学报

外文期刊名：Acta Laboratorium Animalis Scientia Sinica

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家自然科学基金项目(81260520)

语种：中文

中文关键词：溃疡性结肠炎;脾肾阳虚;酶联免疫法;大鼠

外文关键词：Ulcerative colitis; Spleen and kidney Yang deficiency; Enzyme linked immunosorbent assay; Rats

摘要：目的建立脾肾阳虚型溃疡性结肠炎大鼠模型。方法通过灌服大黄水煎液、肌肉注射氢化可的松并结合TNBS(2,4,6-三硝基苯磺酸)混合乙醇灌肠建立脾肾阳虚型UC动物模型。将60只大鼠随机分为空白组、脾肾阳虚型UC模型7、14 d及21 d组,采用酶联免疫法检测各组大鼠血清中FT3、FT4、T的含量。结果与空白组比较,脾肾阳虚型UC模型7、14 d及21 d组大鼠血清中FT3、FT4、T的含量均有降低(P<0.05);尤以模型21 d组差异显著。结论 FT3、FT4、T是脾肾阳虚的敏感指标,检测血清FT3、FT4、T可以更好地佐证脾肾阳虚;证明了脾肾阳虚型UC动物模型复建成功。
Objective To establish a rat model of ulcerative colitis（ UC） with spleen and kidney Yang deficiency. Methods The rat model of ulcerative colitis with spleen and kidney Yang deficiency was established by oral administration of Rhubarb decoction,intramuscular injection of hydrocortisone,and combined with ethanol enema of TNBS（ 2,4,6-trinitrobenzene sulfonic acid）. Sixty Wistar rats（ body weight 180 ± 20 g,male： female = 1 ∶ 1） were randomly divided into blank control group and groups of UC models with spleen kidney Yang deficiency for 7 days,14 days and 21 days. The serum levels of FT3,FT4,and T of the rats were detected by ELISA assay. Results Compared with the blank control group,the serum levels of FT3,FT4,and T in the groups of UC rat models with spleen kidney Yang deficiency for 7 days,14 days and 21 days were decreased to a different extent（ P〈0. 05）,especially,decreased dramatically in the model group for 21 days. Conclusions FT3,FT4,and T are sensitive indexes with spleen and kidney Yang deficiency. The detection of serum levels of FT3,FT4,and T can better verify the spleen and kidney Yang deficiency in the rats,and prove that the spleen and kidney Yang deficiency type UC animal model is successfully prepared.