文献类型：期刊文献

英文题名：Activity-screening-guided isolation and purification for vasodilative effects compounds from Radix Astragali by high-speed counter-current chromatography using gradient elution

作者：Feng, Zu-fei[1,2,3];Chen, Xiao-fen[1,2,3];Zhang, Jia[4];Di, Duo-long[1,2,4]

第一作者：Feng, Zu-fei

通信作者：Di, DL[1]

机构：[1]Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[3]Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China;[4]Chinese Acad Sci, Gansu Coll Tradit Chinese Med, Dept Pharm, Lanzhou 730000, Peoples R China

第一机构：Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China.

年份：2013

卷号：27

期号：11

起止页码：1020

外文期刊名：NATURAL PRODUCT RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000320641900012)】；

基金：This research was supported by the National Science and Technology Support Programme (No. 2007BAI37B05), the National Natural Science Foundation of China (NSFC No.21175142) and the 'Hundred Talents Program' of the Chinese Academy of Sciences (CAS) in 2007.

语种：英文

外文关键词：activity-screening-guided; gradient elution; high-speed counter-current chromatography; Radix Astragali; vasodilative effects

摘要：The animal models were conducted to activity screening of different ethanol fraction from Radix Astragali. Eluted from the macroporous adsorptive resins, the potential active fraction that had vasodilative effects was isolated and purified by high-speed counter-current chromatography (HSCCC) using gradient elution. The solvent system was composed of chloroform-methanol-acetic acid-water (2:1:1:1, v/v/v/v). The flow rate was 1.0mLmin(-1) initially and changed to 2.0mLmin(-1) at 300min. Four flavonoids named calycosin-7-O--D-glycoside (a), formononetin-7-O--D-glycoside (b), calycosin (c) and formononetin (d) with purity over 94% were obtained and then identified by H-1 NMR and C-13 NMR.