文献类型：期刊文献

英文题名：Co-culture with lung cancer A549 cells promotes the proliferation and migration of mesenchymal stem cells derived from bone marrow

作者：Zhang, Yue-Mei[1];Zhang, Zhi-Ming[2,3];Guan, Quan-Lin[1];Liu, Yong-Qi[2,3,4];Wu, Zhi-Wei[2,3,5];Li, Jin-Tian[2,3,4];Su, Yun[1];Yan, Chun-Lu[1];Luo, Ya-Li[1];Qin, Jie[1];Wang, Qian[1];Xie, Xiao-Dong[6]

第一作者：Zhang, Yue-Mei

通信作者：Guan, QL[1];Liu, YQ[2];Liu, YQ[3]

机构：[1]Lanzhou Univ, Hosp 1, Dept Ophthalmol, 1 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China;[2]Prov Level Key Lab Mol Med Major Dis, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China;[3]Prevent & Treatment Tradit Chinese Med Res Gansu, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China;[4]Minist Educ Peoples Republ China, Key Lab Dunhuang Med & Transformat, Beijing, Peoples R China;[5]Gansu Univ Chinese Med, Dept Basic Med Sci, Lanzhou 730000, Gansu, Peoples R China;[6]Lanzhou Univ, Inst Genet, Sch Basic Med Sci, Lanzhou 730000, Gansu, Peoples R China

第一机构：Lanzhou Univ, Hosp 1, Dept Ophthalmol, 1 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Hosp 1, Dept Ophthalmol, 1 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Prov Level Key Lab Mol Med Major Dis, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China;[3]corresponding author), Prevent & Treatment Tradit Chinese Med Res Gansu, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2017

卷号：14

期号：4

起止页码：2983

外文期刊名：EXPERIMENTAL AND THERAPEUTIC MEDICINE

收录：;Scopus(收录号：2-s2.0-85028592271);WOS:【SCI-EXPANDED(收录号:WOS:000410732400034)】；

基金：The present study was supported by the Outstanding Youth Fund Projects of Gansu Province (grant no. 1308RJDA008) and the National Natural Science Foundation of China (grant nos. 81360588 and 81473457).

语种：英文

外文关键词：bone marrow mesenchymal stem cells; lung cancer A549; proliferation; migration; co-culture

摘要：The initiation and progression of various types of tumors, such as lung neoplasms, are driven by a population of cells with stem cell properties and their microenvironment. Bone marrow mesenchymal stem cells (BM-MSCs) in long-term in vitro culture may exhibit spontaneous changes in stem cell biological properties, including malignant transformations; however, the molecular mechanisms of this have not been fully elucidated. In the present study, a BM-MSC and lung cancer A549 cell co-culture system was utilized to investigate how the tumor microenvironment may spontaneously change the proliferation, migration and differentiation of BM-MSCs. It was demonstrated that the lung cancer A549 microenvironment is able to induce changes in the cell morphology, proliferation, karyotype, cytoskeleton and migration ability of BM-MSCs in vitro. Compared with the control group BM-MSCs, the expression of Ras, phosphorylatedextra-cellularregulated protein kinases, nuclear factor-.B, P62 and B-cell lymphoma 2 (Bcl-2) proteins in groups of co-cultured BM-MSCs increased significantly (P<0.05) and the expression of P53, Bcl-2 associated X protein and caspase-3 protein decreased significantly (P<0.05). The mechanisms responsible for the changes observed in BM-MSCs may be related to abnormal expression of related genes in the ERK signaling pathway.