文献类型：期刊文献

英文题名：Single ratio-probe based multichannel sensor integrated with paper-based smartphone platform for pattern recognition of tetracyclines

作者：Lu, Jia-Long[1];Guo, Xin-Ran[1];Wang, Chao[1];Zhang, Ya[1];Li, Lin[2];Chen, Juan[1];Chen, Xin-Yue[1]

第一作者：Lu, Jia-Long

通信作者：Chen, XY[1]

机构：[1]Lanzhou Univ, Inst Pharmaceut Anal, Sch Pharm, Lanzhou 730030, Peoples R China;[2]Gansu Univ Chinese Med, Expt & Training Teaching Ctr, Lanzhou 730000, Peoples R China

第一机构：Lanzhou Univ, Inst Pharmaceut Anal, Sch Pharm, Lanzhou 730030, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Inst Pharmaceut Anal, Sch Pharm, Lanzhou 730030, Peoples R China.

年份：2026

卷号：224

外文期刊名：MICROCHEMICAL JOURNAL

收录：;Scopus(收录号：2-s2.0-105031685449);WOS:【SCI-EXPANDED(收录号:WOS:001710117600001)】；

基金：This work was supported by the Key Laboratory Project for Quality Control of Chinese Medicinal Materials and Decoction Pieces of Gansu Provincial Drug Administration (No. 2025GSMPA-KL02) , the Pharma-ceutical Research Projects in Gansu Province (No. 2025GSMPA067) .

语种：英文

外文关键词：Tetracycline; Smartphone platform; Molecular docking model; Smartphone-assisted testing paper; Multi-channel sensing array; HPS-N; S-CDs; Eu3+

摘要：Tetracycline antibiotics (TCs), including tetracycline (TC), oxytetracycline (OTC), doxycycline (DOX), and chlortetracycline (CTC), are structurally similar but differ in activity and toxicity, necessitating methods for their simultaneous discrimination. While sensing arrays hold promise for the simultaneous identification of multiple analytes, most existing approaches rely on multiple sensing elements and are limited to single-signal output modes. Herein, a ratiometric fluorescence multi-channel sensing array using a single recognition element (HPSN, S-CDs/Eu3+) was developed. Different TCs sensitize Eu3+ variably, producing three characteristic emission peaks under 380 nm excitation. Integrating these with the carbon dots' signal enabled a three-channel ratio array for simultaneous detection and distinction of the four TCs. Molecular docking revealed the structural basis for efficiency variations. A portable system combining paper-based sensors with a smartphone effectively discriminated tetracycline from other antibiotics in spiked milk samples. This strategy provides a robust approach for antibiotic identification and offers new insights into designing ratiometric sensing arrays.