文献类型：期刊文献

中文题名：基于qRT-RNA技术筛选藏药湿生扁蕾抗溃疡性结肠炎肠纤维化活性成分的研究

英文题名：Bioactive constituents studies on Gentianopsis paludosa against UC fibrosis based on the qRT-RNA method

作者：卢年华[1];赵慧巧[1];刘雪枫[1];景明[1];陈正君[1]

第一作者：卢年华

机构：[1]甘肃中医药大学药学院

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2016

卷号：35

期号：11

起止页码：628

中文期刊名：药学研究

外文期刊名：Journal of Pharmaceutical Research

基金：国家自然科学基金项目(No.81360522;No.81560717)

语种：中文

中文关键词：活性成分;qRT-RNA;湿生扁蕾;溃疡性结肠炎

外文关键词：Active component ; qRT-RNA ; Gentianopsis paludosa ; Ulcerative colitis

摘要：目的研究藏药湿生扁蕾抗溃疡性结肠炎肠纤维化的药效物质基础。方法采用2,4,6-三硝基苯磺酸(TNBS)诱导的大鼠溃疡性结肠炎肠纤维化模型,灌胃给予藏药湿生扁蕾用乙酸乙酯萃取的活性提取物,以硅胶色谱分离、薄层定性得到的活性部位,采用荧光定量RT-PCR法检测大鼠结肠组织胶原ⅠmRNA、胶原ⅢmRNA、α-SMA mRNA、E-cad mRNA的表达,筛选其活性成分。结果湿生扁蕾乙酸乙酯萃取物分离得到的活性部位经纯化得到4个单体化合物,用光谱分析等鉴定为1-hydroxy-3,7,8-Trimethoxyxanthones、1,8-Dihydroxy-3,7-Dimethoxyxanthones、1-hydroxy-3,7-Dimethoxyxanthones和1,7-Dihydroxy-3,8-Dimethoxyxanthones,4种单体化合物对溃疡性结肠炎肠纤维化大鼠模型均有明显的活性。结论湿生扁蕾抗溃疡性结肠炎肠纤维化的物质基础集中在乙酸乙酯部位,活性成分主要体现为4种酮类化合物。
Objective To study the bioactive constituents of Tibetan medicine Gentianopsis paludosa against UC fibro-sis.Methods Active fragment were obtained by silica gel and TLC from ethyl acetate active part which was extracted fromTibetan Gentianopsis paludosa.By method of qRT-PCR, collagen I , collagen m, c^-SMA, and E-cad were detected toscreen the active components after model rats being treated with active isolates.Results After purified and identified fromethyl acetate part using spectroscopy, four monomer compounds （ 1 -hydroxy- 3,7,8-Trimethoxyxanthones ; 1,8-Dihydroxy-3,7-Dimethoxyxanthones ; 1-hydroxy-3,7- Dimethoxyxanthones and 1,7- Dihydroxy-3,8- Dimethoxyxanthones ） had sig-nificant bioactive on UC fibrosis.Conclusion The material basis of Gentianopsis paludosa against fibrosis concentrated inparts of ethyl acetate, and the active ingredient mainly owed to four xanthones.