文献类型：期刊文献

中文题名：红芪多糖对糖尿病肾病db/db小鼠肾功能和肾脏组织中GluT-1 mRNA和蛋白表达水平的影响

英文题名：Influence of hedysaryum polysaccharide in kidney function and expressions of Glut-1 mRNA and protein in kidney tissue of db/db mice with diabetic nephropathy

作者：金智生[1];朱真灵[1];魏玉娇[1];郑丽红[2];关燕[1];齐雪艳[1]

第一作者：金智生

机构：[1]甘肃中医学院中医医疗系,甘肃兰州730000;[2]黑龙江中医药大学中医医疗系,黑龙江哈尔滨150040

第一机构：甘肃中医药大学中医临床学院

年份：2014

卷号：40

期号：3

起止页码：519

中文期刊名：吉林大学学报（医学版）

外文期刊名：Journal of Jilin University：Medicine Edition

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家自然科学基金资助课题(81160427)

语种：中文

中文关键词：红芪多糖;糖尿病肾病;葡萄糖转运蛋白-1;db;db小鼠

外文关键词：hedysaryum polysaccharide;diabetic nephropathy;db/db mice

摘要：目的:探讨红芪多糖对糖尿病肾病(DN)db/db小鼠肾功能和肾脏组织中葡萄糖转运蛋白-1(GluT-1)的mRNA和蛋白表达的影响,阐明其可能的作用机制。方法:将10只db/m小鼠作为正常对照组;50只肥胖型2型糖尿病模型db/db小鼠随机分为模型组,依那普利组,红芪多糖低、中和高剂量组;每组10只。正常对照组和模型组小鼠分别灌胃给予生理盐水0.5 mL·kg-1·d-1,其他各组小鼠分别灌胃给予依那普利10mg·kg-1·d-1和红芪多糖100、200和400mg·kg-1·d-1,共8周。苦味酸法测定小鼠血肌酐(SCr)水平,酶偶联速率法测定血尿素氮(BUN)水平,ELISA法测定24h尿微量白蛋白(UMALB)水平;RT-PCR法测定小鼠肾脏组织中GluT-1mRNA的表达;Western blotting法和免疫组织化学法测定小鼠肾脏组织中GluT-1蛋白的表达。结果:与模型组比较,依那普利组和红芪多糖各剂量组SCr、BUN、UMALB和GluT-1mRNA及蛋白表达水平均降低,其中红芪多糖高剂量组和依那普利组降低明显(P<0.01)。HE及Masson染色,红芪多糖高剂量组小鼠肾小球内炎症细胞浸润较少,毛细血管腔通畅,肾小管及肾间质内胶原蛋白沉积不明显。结论:红芪多糖可改善db/db小鼠的肾功能并明显抑制GluT-1mRNA和蛋白的表达,提示红芪多糖可能通过抑制肾小球系膜细胞膜上GluT-1的表达而延缓DN的发展。
Objective To investigate the influence of hedysaryum polysaccharide （HPS）in the kidney function and expressions of Glut-1 mRNA and protein in kidney tissue of db/db mice with diabetic nephropathy （DN）and to elucidate its possible action mechanism.Methods 10 db/m mice were taken as normal control group（n=10）;50 fueling animal model db/db mice with DN were randomly divided into model group,enalapril group and the low, middle and high doses of HPS groups（n=10）.The mice in noral control group and model group were given physioloical saline by gavege;and the mice in the other groups were respectively given 10 mg·kg-1 ·d-1 enalapril, 100,200 and 400 mg·kg-1 ·d-1 HPS by gavage;lasted 8 weeks.Picric acid method was used to determine the＆amp;nbsp;serum creatinine（SCr）level of the mice,enzyme coupling rate method was used to determine the blood urea nitrogen （BUN）level,ELISA method was used to determine the urinary microalbumin（UMALB）level,RT-PCR method was performed to detect the expression of Glut-1 mRNA, and Western blotting and immunohistochemical methods were used to detect the expression of Glut-1 protein.Results Compared with model group,the levels of SCr, BUN, UMALB, the mRNA and protein of Glut-1 expressions were decreased, especially in 400 mg·kg-1 ·d-1 HPS and enalapril groups（P〈0.01）.The HE and Masson staining results showed that less inflammatory cells infiltration in glomerular of the mice were found, capillary lumens were unobstructed, and the collagen deposition was not obvious in 400 mg·kg-1 ·d-1 group.Conclusion HPS could improve the kidney function of the db/db mice and inhibit the Glut-1 mRNA and protein expressions obviously, which indicates that HPS could delay the development of DN by inhibiting the Glut-1 expression in the glomerular mesangial cell membrane.