文献类型：期刊文献

中文题名：补肾通络方抑制NF-κB/RANK/RANKL通路减轻胶原蛋白诱导关节炎(CIA)大鼠骨破坏

英文题名：Bushentongluo recipe(BSTL)attenuates bone destruction by inhibiting NF-κB/RANK/RANKL pathway in collagen-induced arthritis(CIA)rats

作者：刘海龙[1];王钢[1];王佳[1];王涛[1];田杰祥[1];王丽琴[1];杨芳[1]

第一作者：刘海龙

机构：[1]甘肃中医药大学附属医院风湿骨病科,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2021

卷号：37

期号：3

起止页码：205

中文期刊名：细胞与分子免疫学杂志

外文期刊名：Chinese Journal of Cellular and Molecular Immunology

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；PubMed;

基金：国家自然科学基金(81860858)。

语种：中文

中文关键词：补肾通络方(BSTL);类风湿性关节炎(RA);核因子κB受体激活蛋白配体(RANKL);核因子κB p65(NF-κB p65);胶原蛋白诱导关节炎(CIA)

外文关键词：Bushentongluo recipe(BSTL);rheumatoid arthritis(RA);receptor activator for nuclear factor-κB ligand(RANKL);nuclear factor-κB p65(NF-κB p65);collagen-induced arthritis(CIA)

摘要：目的探讨补肾通络方(BSTL)对胶原蛋白诱导关节炎(CIA)大鼠模型骨破坏的改善作用以及对核因子κB/核因子κB受体激活蛋白/核因子κB受体激活蛋白配体(NF-κB/RANK/RANKL)信号通路的影响。方法将SD大鼠随机分为空白对照组、CIA模型组、1 mg/kg甲氨喋呤(MTX)处理组、(0.5、2)g/kgBSTL处理组,每组10只。除空白对照组外,其余大鼠采用含有卡介苗的完全Freund佐剂和牛Ⅱ型胶原蛋白(Col2)混合乳液免疫刺激,建立CIA模型。建模15 d后(第0天)根据关节炎指数(AI)评价造模是否成功。自第0天起,连续给予MTX或BSTL处理28 d,免疫组织化学染色法检测滑膜组织NF-κB p65的表达,ELISA检测大鼠血清白细胞介素1β(IL-1β)、IL-18、肿瘤坏死因子α(TNF-α)、抗总Col2抗体及亚型(Col2-IgG、Col2-IgG1、Col2-IgG2a)水平,Western blot法检测滑膜组织RANKL、RANK、护骨因子(OPG)蛋白表达。结果与CIA模型组相比,2 g/kg BSTL处理28 d的大鼠足容积、AI值降低,血清IL-1β、IL-18、TNF-α、Col2-IgG、Col2-IgG2a以及RANK和RANKL水平均降低,OPG水平升高;大鼠滑膜组织中NF-κB p65、RANK和RANKL蛋白表达均降低,OPG蛋白表达增加。结论BSTL可通过抑制全身炎症反应,降低滑膜组织中NF-κB p65、RANK、RANKL蛋白的表达,上调OPG蛋白表达,缓解CIA模型大鼠关节炎症和肿胀。
Objective To investigate the therapeutic effect of Bushentongluo recipe(BSTL)on bone destruction and its inhibiting effect on NF-κB/RANK/RANKL pathway in collagen-induced arthritis(CIA)rats.Methods SD rats were randomly divided into blank control group,CIA model group,methotrexate(MTX,1 mg/kg)group,BSTL 0.5 g/kg and 2 g/kg groups,with 10 rats in each.Except the control group,the other rats were injected subcutaneously with type 2 collagen(Col2)at the base of the tail to establish CIA models.After exposure to MTX or BSTL recipe for consecutive 28 days,the pathological change of joint tissues was examined by HE staining.Immunohistochemistry was used to detect NF-κB p65 expression in synovial tissues.The cytokines and anti-Col2 levels were analyzed by ELISA.Western blotting was used to detect the expression of RANKL,RANK and osteoprotegerin(OPG)proteins.Results Compared with the CIA model group,the rats treated with 2 g/kg BSTL for 28 days had lower paw volume,arthritis index(AI),serum levels of IL-1β,IL-18,TNF-α,anti-Col2-IgG,anti-Col2-IgG2a,RANK and RANKL,and higher level of serum OPG.Besides,Western blotting showed that the expression of NF-κB p65,RANK and RANKL proteins decreased,but the expression of OPG protein increased in BSTL 2 g/kg group.Conclusion BSTL can alleviate the rheumatoid arthritis by down-regulating the expression of NF-κB p65,RANKL,RANK proteins,up-regulating OPG protein,and inhibiting systemic inflammation.