文献类型：期刊文献

英文题名：Study on the structure of root nodules of Hedysarum polybotrys Hand.-Mazz. and the isolation and identification of rhizobia

作者：Liang, Tingting[1];Tan, Xueyan[1];Zhang, Guangmao[1];Li, Xinrong[1];Qiang, Zhengze[1];Fu, Kairun[1];Luo, Xudong[1];Li, Chengyi[1]

第一作者：梁婷婷

通信作者：Li, CY[1]

机构：[1]Gansu Univ Chinese Med, Sch Pharm, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Pharm, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：42

期号：4

起止页码：431

外文期刊名：PLANT BIOTECHNOLOGY

收录：;Scopus(收录号：2-s2.0-105027134551);WOS:【SCI-EXPANDED(收录号:WOS:001651175300001)】；

语种：英文

外文关键词：Hedysarum polybotrys; nodule structure; promote growth; rhizobium; root nodules

摘要：Hedysari Radix, a significant Chinese herbal medicine from Northwest China's arid region, is renowned for its unique tonic effects in traditional Chinese medicine practices. This plant, a member of the Leguminosae family, forms a symbiotic relationship with nitrogen-fixing rhizobia. However, the Hedysarum polybotrys-rhizobium symbiotic system remains underexplored. The root nodule structure of H. polybotrys was examined using an optical microscope (OM). This examination revealed that its root nodules consist of meristematic zone, infection zone, nitrogen fixation zone, and senescence zone, arranged from top to bottom. This structure suggests that the root nodules of H. polybotrys belong to the indeterminate nodule category. In the fields of transmission electron microscopy (TEM) and fields emission scanning electron microscopy (FESEM), significant differences were observed between infected and un-infected cells. Rhizobium, identified via 16S rRNA technology and classified as the genus Mesorhizobium through phylogenetic analysis. Reinoculation of rhizobium into H. polybotrys seedlings resulted in nodule formation on the roots. Notably, inoculated plants exhibited a considerable increase in nodule number, leaf count, leaf length, aboveground height, aboveground fresh weight, root length, and root diameter compared to uninoculated controls, demonstrating that rhizobium inoculation enhances plant growth.